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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NAT10 Recombinant antibody (82585-1-RR)

    WB analysis using 82585-1-RR (same clone as 82585-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82585-1-RR (NAT10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82585-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using NAT10 Recombinant antibody (82585-1-RR)

    WB analysis of HeLa using 82585-1-RR (same clone as 82585-1-PBS)

    WB result of NAT10 antibody (82585-1-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NAT10 transfected HeLa cells. This data was developed using the same antibody clone with 82585-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using NAT10 Recombinant antibody (82585-1-RR)

    IP experiment of mouse testis using 82585-1-RR (same clone as 82585-1-PBS)

    IP result of anti-NAT10 (IP:82585-1-RR, 4ug; Detection:82585-1-RR 1:5000) with mouse testis tissue lysate 1120 ug. This data was developed using the same antibody clone with 82585-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NAT10 Recombinant antibody (82585-1-RR)

    IF Staining of HeLa using 82585-1-RR (same clone as 82585-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NAT10 antibody (82585-1-RR, Clone: 5B9 ) at dilution of 1:1900 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 82585-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NAT10 Recombinant antibody (82585-1-RR)

    IF Staining of HeLa using 82585-1-RR (same clone as 82585-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NAT10 antibody (82585-1-RR, Clone: 5B9 ) at dilution of 1:1900 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 82585-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82585-1-RR

    Affinity and Kinetic Characterization of 82585-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82585-1-RR against Human NAT10 were performed. The affinity constant is below 1 pM.

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  • Featured Product
  • KD/KO Validated

NAT10 Recombinant antibody, PBS Only

NAT10 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 82585-1-PBS
Clone No.5B9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

N acetyltransferase 10, KIAA1709, EC:2.3.1.-, ALP, 5B9

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82585-1-PBS targets NAT10 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NAT10 fusion protein Ag4184 相同性解析による交差性が予測される生物種
Full Name N-acetyltransferase 10 (GCN5-related)
Calculated molecular weight 1025 aa, 116 kDa
Observed molecular weight 116 kDa
GenBank accession numberBC035558
Gene Symbol NAT10
Gene ID (NCBI) 55226
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9H0A0
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

NAT10 (N-acetyltransferase 10) is a nucleolar protein that is involved in regulation of telomerase activity, DNA damage response, and cytokinesis. It also plays a role in maintaining nuclear shape. Inhibition of NAT10 has been reported to rescue the misshapen nuclei in laminopathic cells via microtubule reorganization. The specificity of this antibody has been tested by siRNA (PMID: 24786082). NAT10 regulates mitotic cell fate by acetylating Eg5. NAT10 depletion results in multinuclear giant cells, which is the hallmark of mitotic catastrophe (PMID: 35210604). NAT10 plays a crucial role in carcinogenesis through influencing EMT, hypoxia, ribosomal biogenesis and overall, promoting translational efficiency. Recently, we reported that treating cancer cells with Remodelin, a small molecule inhibitor of NAT10, causes alteration in global lipid metabolism (PMID: 36149760).