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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NAT10 Recombinant antibody

NAT10 Recombinant Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

5B9

Cat no : 82585-1-RR

Synonyms

N acetyltransferase 10, KIAA1709, EC:2.3.1.-, ALP, 5B9

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NAT10 Recombinant antibody (82585-1-RR)

    WB analysis using 82585-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82585-1-RR (NAT10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using NAT10 Recombinant antibody (82585-1-RR)

    IP experiment of mouse testis using 82585-1-RR

    IP result of anti-NAT10 (IP:82585-1-RR, 4ug; Detection:82585-1-RR 1:5000) with mouse testis tissue lysate 1120 ug.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NAT10 Recombinant antibody (82585-1-RR)

    IF Staining of HeLa using 82585-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NAT10 antibody (82585-1-RR, Clone: 5B9 ) at dilution of 1:1900 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NAT10 Recombinant antibody (82585-1-RR)

    IF Staining of HeLa using 82585-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NAT10 antibody (82585-1-RR, Clone: 5B9 ) at dilution of 1:1900 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

  • Affinity and Kinetic Characterization of 82585-1-RR

    Affinity and Kinetic Characterization of 82585-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82585-1-RR against Human NAT10 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inHeLa cells, A549 cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive IP detected inmouse testis tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:1500-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82585-1-RR targets NAT10 in WB, IF/ICC, IP, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NAT10 fusion protein Ag4184 相同性解析による交差性が予測される生物種
Full Name N-acetyltransferase 10 (GCN5-related)
Calculated molecular weight 1025 aa, 116 kDa
Observed molecular weight 116 kDa
GenBank accession numberBC035558
Gene symbol NAT10
Gene ID (NCBI) 55226
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NAT10 (N-acetyltransferase 10) is a nucleolar protein that is involved in regulation of telomerase activity, DNA damage response, and cytokinesis. It also plays a role in maintaining nuclear shape. Inhibition of NAT10 has been reported to rescue the misshapen nuclei in laminopathic cells via microtubule reorganization. The specificity of this antibody has been tested by siRNA (PMID: 24786082). NAT10 regulates mitotic cell fate by acetylating Eg5. NAT10 depletion results in multinuclear giant cells, which is the hallmark of mitotic catastrophe (PMID: 35210604). NAT10 plays a crucial role in carcinogenesis through influencing EMT, hypoxia, ribosomal biogenesis and overall, promoting translational efficiency. Recently, we reported that treating cancer cells with Remodelin, a small molecule inhibitor of NAT10, causes alteration in global lipid metabolism (PMID: 36149760).

Protocols

Product Specific Protocols
WB protocol for NAT10 antibody 82585-1-RRDownload protocol
IF protocol for NAT10 antibody 82585-1-RRDownload protocol
IP protocol for NAT10 antibody 82585-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols