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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NAT6/FUS2 Polyclonal antibody (15476-1-AP)

    WB analysis using 15476-1-AP (same clone as 15476-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15476-1-AP (NAT6/FUS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15476-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NAT6/FUS2 Polyclonal antibody (15476-1-AP)

    IF Staining of HeLa using 15476-1-AP (same clone as 15476-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using NAT6/FUS2 antibody (15476-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15476-1-PBS in a different storage buffer formulation.

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  • KD/KO Validated

NAT6/FUS2 Polyclonal antibody, PBS Only

NAT6/FUS2 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 15476-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15476-1-PBS targets NAT6/FUS2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NAT6/FUS2 fusion protein Ag7780 相同性解析による交差性が予測される生物種
Full Name N-acetyltransferase 6 (GCN5-related)
Calculated molecular weight 31 kDa
Observed molecular weight45 kDa
GenBank accession numberBC004483
Gene Symbol FUS2
Gene ID (NCBI) 24142
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ93015
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

The acetyltransferase NAT6 (also known as Fus2) is an enzyme that catalyzes the transfer of acetyl groups from acetyl-CoA to acrylamines and located mainly in the cytoplasm. NAT6 has two isoforms. The molecular weight of NAT6 was detected to be about 45kDa (PMID:32978259).