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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated NCAM1/CD56 Monoclonal antibody

NCAM1/CD56 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1E8C9

Cat no : CL488-60238

Synonyms

CD56, MSK39, N CAM 1, NCAM, NCAM 1, NCAM1, NCAM1/CD56

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using CoraLite® Plus 488-conjugated NCAM1/CD56 Monoclona (CL488-60238)

    IF Staining of SH-SY5Y using CL488-60238

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using CoraLite® Plus 488 NCAM1/CD56 antibody (CL488-60238, Clone: 1E8C9 ) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-60238 targets NCAM1/CD56 in IF/ICC applications and shows reactivity with human, mouse, pig, rat samples.

Tested Reactivity human, mouse, pig, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NCAM1/CD56 fusion protein Ag5732 相同性解析による交差性が予測される生物種
Full Name neural cell adhesion molecule 1
Calculated molecular weight 95 kDa
Observed molecular weight 140 kDa
GenBank accession numberBC047244
Gene symbol NCAM1
Gene ID (NCBI) 4684
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Neural cell adhesion molecule 1 (NCAM1, also known as CD56) is a cell adhesion glycoprotein of the immunoglobulin (Ig) superfamily. It is a multifunction protein involved in synaptic plasticity, neurodevelopment, and neurogenesis. NCAM1 is expressed on human neurons, glial cells, skeletal muscle cells, NK cells and a subset of T cells, and the expression is observed in a wide variety of human tumors, including myeloma, myeloid leukemia, neuroendocrine tumors, Wilms' tumor, neuroblastoma, and NK/T cell lymphomas. Three major isoforms of NCAM1, with molecular masses of 120, 140, and 180 kDa, are generated by alternative splicing of mRNA (PMID: 9696812). The glycosylphosphatidylinositol (GPI)-anchored NCAM120 and the transmembrane NCAM140 and NCAM180 consist of five Ig-like domains and two fibronection-type III repeats (FNIII). All three forms can be posttranslationally modified by addition of polysialic acid (PSA) (PMID: 14976519). Several other isofroms have also been described (PMID: 1856291).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 NCAM1/CD56 antibody CL488-60238Download protocol
Standard Protocols
Click here to view our Standard Protocols