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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NCAPG Monoclonal antibody

NCAPG Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

2D4D3

Cat no : 68082-1-Ig

Synonyms

CAPG, CHCG, Condensin complex subunit 3, Condensin subunit CAP G, FLJ12450, HCAP G, Melanoma antigen NY MEL 3, NCAPG, NY MEL 3, NYMEL3, XCAP G homolog

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NCAPG Monoclonal antibody (68082-1-Ig)

    WB analysis using 68082-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68082-1-Ig (NCAPG antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using NCAPG Monoclonal antibody (68082-1-Ig)

    IF Staining of U2OS using 68082-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using NCAPG antibody (68082-1-Ig, Clone: 2D4D3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of U2OS cells using NCAPG Monoclonal antibody (68082-1-Ig)

    FC experiment of U2OS using 68082-1-Ig

    1X10^6 U2OS cells were intracellularly stained with 0.4 ug Anti-Human NCAPG (68082-1-Ig, Clone:2D4D3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inA431 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inU2OS cells
Positive FC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68082-1-Ig targets NCAPG in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen NCAPG fusion protein Ag19926 相同性解析による交差性が予測される生物種
Full Name non-SMC condensin I complex, subunit G
Calculated molecular weight 1015 aa, 114 kDa
Observed molecular weight114 kDa
GenBank accession numberBC068467
Gene symbol NCAPG
Gene ID (NCBI) 64151
RRIDAB_2918819
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NCAPG antibody 68082-1-IgDownload protocol
IF protocol for NCAPG antibody 68082-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols