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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NCAPG Monoclonal antibody (68082-1-Ig)

    WB analysis using 68082-1-Ig (same clone as 68082-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68082-1-Ig (NCAPG antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control. This data was developed using the same antibody clone with 68082-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using NCAPG Monoclonal antibody (68082-1-Ig)

    IF Staining of U2OS using 68082-1-Ig (same clone as 68082-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using NCAPG antibody (68082-1-Ig, Clone: 2D4D3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68082-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of U2OS cells using NCAPG Monoclonal antibody (68082-1-Ig)

    FC experiment of U2OS using 68082-1-Ig (same clone as 68082-1-PBS)

    1X10^6 U2OS cells were intracellularly stained with 0.4 ug Anti-Human NCAPG (68082-1-Ig, Clone:2D4D3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68082-1-PBS in a different storage buffer formulation.

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NCAPG Monoclonal antibody, PBS Only

NCAPG Monoclonal Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 68082-1-PBS
Clone No.2D4D3

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

CAPG, CHCG, Condensin complex subunit 3, Condensin subunit CAP G, FLJ12450, HCAP G, Melanoma antigen NY MEL 3, NCAPG, NY MEL 3, NYMEL3, XCAP G homolog

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68082-1-PBS targets NCAPG in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen NCAPG fusion protein Ag19926 相同性解析による交差性が予測される生物種
Full Name non-SMC condensin I complex, subunit G
Calculated molecular weight 1015 aa, 114 kDa
Observed molecular weight114 kDa
GenBank accession numberBC068467
Gene Symbol NCAPG
Gene ID (NCBI) 64151
RRIDAB_2918819
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9BPX3
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.