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Species Reactivity

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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated NCAPH Monoclonal antibody

NCAPH Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF-P

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

3D2F11

Cat no : CL647-67655

Synonyms

Barren homolog protein 1, BRRN, BRRN1, CAP H, CAPH, Condensin complex subunit 2, HCAP H, KIAA0074, NCAPH, XCAP H homolog

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using CoraLite® Plus 647-conjugated NCAPH Monoclonal ant (CL647-67655)

    IF Staining of human liver cancer using CL647-67655

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite® Plus 647 NCAPH antibody (CL647-67655, Clone: 3D2F11 ) at dilution of 1:200.

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Tested Applications

Positive IF-P detected inhuman liver cancer tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-67655 targets NCAPH in IF-P applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NCAPH fusion protein Ag27748 相同性解析による交差性が予測される生物種
Full Name non-SMC condensin I complex, subunit H
Calculated molecular weight 741 aa, 83 kDa
GenBank accession numberBC024211
Gene symbol NCAPH
Gene ID (NCBI) 23397
RRIDAB_2920297
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Non-SMC condensin I complex subunit H (NCAPH) is one of the three non-SMC subunits in condensin I, which belongs to a recently defined superfamily of proteins termed kleisins. Another two non-SMC subunits, CAP-D2 and CAP-G, share a highly degenerate repeating motif known as HEAT repeat. Some studies show that each subunit is essential for viability and plays an important role in mitotic chromosome architecture and segregation. In recent years, researchers found that the high expression of NCAPH was associated with poor prognosis in patients with non-small cell lung cancer and prostate cancer. Downregulation of NCAPH inhibited the proliferation, migration, and invasion of several cancer cells significantly. Moreover, NCAPH was involved in the regulation of mature chromosome condensation and DNA damage. These data suggest that NCAPH may be a key carcinogen involved in the development and progression of human malignant tumors. ( PMID: 28300828, PMID: 33311486 )

Protocols

Product Specific Protocols
IF protocol for CL Plus 647 NCAPH antibody CL647-67655Download protocol
Standard Protocols
Click here to view our Standard Protocols