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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using Nucleolin/C23 Recombinant antibody (83380-1-RR)

    WB analysis of HeLa using 83380-1-RR (same clone as 83380-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83380-1-RR (Nucleolin/C23 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83380-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using Nucleolin/C23 Recombinant antibody (83380-1-RR)

    IP experiment of HeLa using 83380-1-RR (same clone as 83380-1-PBS)

    IP result of anti-Nucleolin/C23 (IP:83380-1-RR, 4ug; Detection:83380-1-RR 1:5000) with HeLa cells lysate 1085 ug. This data was developed using the same antibody clone with 83380-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Nucleolin/C23 Recombinant antibody (83380-1-RR)

    IHC staining of human lung cancer using 83380-1-RR (same clone as 83380-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 83380-1-RR (Nucleolin/C23 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83380-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Nucleolin/C23 Recombinant antibody (83380-1-RR)

    IHC staining of human lung cancer using 83380-1-RR (same clone as 83380-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 83380-1-RR (Nucleolin/C23 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83380-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Nucleolin/C23 Recombinant antibody (83380-1-RR)

    IF Staining of HepG2 using 83380-1-RR (same clone as 83380-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Nucleolin/C23 antibody (83380-1-RR, Clone: 240029F8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83380-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HEK-293 cells using Nucleolin/C23 Recombinant antibody (83380-1-RR)

    FC experiment of HEK-293 using 83380-1-RR (same clone as 83380-1-PBS)

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug Nucleolin/C23 Recombinant antibody (83380-1-RR, Clone:240029F8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83380-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83380-1-RR

    Affinity and Kinetic Characterization of 83380-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83380-1-RR against Human Nucleolin/C23 were performed. The affinity constant is 2.47 nM.

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Nucleolin/C23 Recombinant antibody, PBS Only

Nucleolin/C23 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 83380-1-PBS
Clone No.240029F8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Synonyms

C23, nucleolin, Protein C23, NCL, FLJ45706

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83380-1-PBS targets Nucleolin/C23 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Nucleolin/C23 fusion protein Ag0859 相同性解析による交差性が予測される生物種
Full Name nucleolin
Calculated molecular weight 76 kDa
Observed molecular weight 100-110 kDa
GenBank accession numberBC006494
Gene Symbol NCL
Gene ID (NCBI) 4691
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP19338
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Nucleolin, also known as C23, involved in the control of transcription of ribosomal RNA (rRNA) genes by RNA polymerase I, in nucleocytoplasmic transportation of ribosomal components, and in ribosome maturation and assembly. It associated with intranucleolar chromatin and pre-ribosomal particles, and induced chromatin decondensation by binding to histione H1. Also it has a role in the process of transcriptional elongation. Whilst mammalian nucleolin has a predicted molecular mass of approximately 77 kDa, the apparent molecular mass is between 100 and 110 kDa, and has been attributed to the amino acid composition of the N-terminal domain, which is highly phosphorylated. (PMID: 15925566)