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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Nucleolin/C23 Recombinant antibody

Nucleolin/C23 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

CloneNo.

240029F8

Cat no : 83380-1-RR

Synonyms

C23, nucleolin, Protein C23, NCL, FLJ45706

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using Nucleolin/C23 Recombinant antibody (83380-1-RR)

    WB analysis of HeLa using 83380-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83380-1-RR (Nucleolin/C23 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using Nucleolin/C23 Recombinant antibody (83380-1-RR)

    IP experiment of HeLa using 83380-1-RR

    IP result of anti-Nucleolin/C23 (IP:83380-1-RR, 4ug; Detection:83380-1-RR 1:5000) with HeLa cells lysate 1085 ug.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Nucleolin/C23 Recombinant antibody (83380-1-RR)

    IHC staining of human lung cancer using 83380-1-RR

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 83380-1-RR (Nucleolin/C23 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Nucleolin/C23 Recombinant antibody (83380-1-RR)

    IHC staining of human lung cancer using 83380-1-RR

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 83380-1-RR (Nucleolin/C23 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Nucleolin/C23 Recombinant antibody (83380-1-RR)

    IF Staining of HepG2 using 83380-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Nucleolin/C23 antibody (83380-1-RR, Clone: 240029F8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HEK-293 cells using Nucleolin/C23 Recombinant antibody (83380-1-RR)

    FC experiment of HEK-293 using 83380-1-RR

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug Nucleolin/C23 Recombinant antibody (83380-1-RR, Clone:240029F8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83380-1-RR

    Affinity and Kinetic Characterization of 83380-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83380-1-RR against Human Nucleolin/C23 were performed. The affinity constant is 2.47 nM.

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Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, MCF-7 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83380-1-RR is Nucleolin/C23 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Nucleolin/C23 fusion protein Ag0859 相同性解析による交差性が予測される生物種
Full Name nucleolin
Calculated molecular weight 76 kDa
Observed molecular weight 100-110 kDa
GenBank accession numberBC006494
Gene symbol NCL
Gene ID (NCBI) 4691
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Nucleolin, also known as C23, involved in the control of transcription of ribosomal RNA (rRNA) genes by RNA polymerase I, in nucleocytoplasmic transportation of ribosomal components, and in ribosome maturation and assembly. It associated with intranucleolar chromatin and pre-ribosomal particles, and induced chromatin decondensation by binding to histione H1. Also it has a role in the process of transcriptional elongation. Whilst mammalian nucleolin has a predicted molecular mass of approximately 77 kDa, the apparent molecular mass is between 100 and 110 kDa, and has been attributed to the amino acid composition of the N-terminal domain, which is highly phosphorylated. (PMID: 15925566)

Protocols

Product Specific Protocols
WB protocol for Nucleolin/C23 antibody 83380-1-RRDownload protocol
IHC protocol for Nucleolin/C23 antibody 83380-1-RRDownload protocol
IF protocol for Nucleolin/C23 antibody 83380-1-RRDownload protocol
IP protocol for Nucleolin/C23 antibody 83380-1-RRDownload protocol
FC protocol for Nucleolin/C23 antibody 83380-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols