• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HeLa cells using Kiz Polyclonal antibody (21177-1-AP)

    WB analysis of HeLa using 21177-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 21177-1-AP (C20orf19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using Kiz Polyclonal antibody (21177-1-AP)

    WB analysis of human brain using 21177-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 21177-1-AP (C20orf19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using Kiz Polyclonal antibody (21177-1-AP)

    IP experiment of HeLa using 21177-1-AP

    IP result of anti-Kiz (IP:21177-1-AP, 3ug; Detection:21177-1-AP 1:500) with HeLa cells lysate 2000ug.

  • Immunohistochemistry (IHC) staining of human testis tissue using Kiz Polyclonal antibody (21177-1-AP)

    IHC staining of human testis using 21177-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 21177-1-AP (C20orf19 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using Kiz Polyclonal antibody (21177-1-AP)

    IHC staining of human testis using 21177-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 21177-1-AP (C20orf19 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Kiz Polyclonal antibody (21177-1-AP)

    IF Staining of HeLa using 21177-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Kiz antibody (21177-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), Gamma Tubulin antibody (66320-1-Ig, Clone: 3F9H8, red).

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using Kiz Polyclonal antibody (21177-1-AP)

    IF Staining of hTERT-RPE1 using 21177-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using Kiz antibody (21177-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

Kiz Polyclonal antibody

Kiz Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 21177-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

C20orf19, Centrosomal protein kizuna, HT013, KIZ, NCRNA00153, non protein coding RNA 153, PLK1S1, Polo like kinase 1 substrate 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, human brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, hTERT-RPE1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 2 publications below

Product Information

21177-1-AP targets Kiz in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Kiz fusion protein Ag15454 相同性解析による交差性が予測される生物種
Full Name non-protein coding RNA 153
Calculated molecular weight 673 aa, 75 kDa
Observed molecular weight 90-100 kDa
GenBank accession numberBC045826
Gene Symbol Kiz
Gene ID (NCBI) 55857
RRIDAB_10755292
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ2M2Z5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Kiz antibody 21177-1-APDownload protocol
IHC protocol for Kiz antibody 21177-1-APDownload protocol
IF protocol for Kiz antibody 21177-1-APDownload protocol
IP protocol for Kiz antibody 21177-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Science

A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes.

Authors - Chun So
View Article
humanIF

Science

The mechanism of acentrosomal spindle assembly in human oocytes

Authors - Tianyu Wu
View Article