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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 647-conjugated NCS1 Monoclonal antibody

NCS1 Monoclonal Antibody for WB, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, pig

Applications

WB, FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

2F10C8

Cat no : CL647-67616

Synonyms

DKFZp761L1223, FLUP, FREQ, Frequenin homolog, frequenin homolog (Drosophila), Frequenin like protein, NCS 1, NCS1, Neuronal calcium sensor 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CoraLite® Plus 647-conjugated NCS1 Monoclonal anti (CL647-67616)

    WB analysis using CL647-67616

    Various lysates were subjected to SDS PAGE followed by western blot with CL647-67616 (NCS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of SH-SY5Y cells using CoraLite® Plus 647-conjugated NCS1 Monoclonal anti (CL647-67616)

    FC experiment of SH-SY5Y using CL647-67616

    1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-Human NCS1 (CL647-67616, Clone:2F10C8) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue, pig brain tissue
Positive FC (Intra) detected inSH-SY5Y cells
Positive FC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-67616 targets NCS1 in WB, FC (Intra) applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity Human, mouse, rat, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen NCS1 fusion protein Ag29808 相同性解析による交差性が予測される生物種
Full Name frequenin homolog (Drosophila)
Calculated molecular weight 22 kDa
Observed molecular weight 20 kDa
GenBank accession numberBC004856
Gene symbol NCS1
Gene ID (NCBI) 23413
RRIDAB_2920295
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CL Plus 647 NCS1 antibody CL647-67616Download protocol
Standard Protocols
Click here to view our Standard Protocols