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NDE1 Polyclonal antibody
NDE1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 10233-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A2780 cells, HEK-293 cells, HeLa cells, rat liver tissue, mouse liver tissue, HepG2 cells, mouse brain tissue, rat brain tissue |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human breast cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human breast cancer tissue |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 9 publications below |
| WB | See 25 publications below |
| IHC | See 2 publications below |
| IF | See 20 publications below |
| IP | See 1 publications below |
| CoIP | See 2 publications below |
Product Information
10233-1-AP targets NDE1 in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | NDE1 fusion protein Ag0336 相同性解析による交差性が予測される生物種 |
| Full Name | nudE nuclear distribution gene E homolog 1 (A. nidulans) |
| Calculated molecular weight | 335 aa, 38 kDa |
| Observed molecular weight | 40 kDa |
| GenBank accession number | BC001421 |
| Gene symbol | NDE1 |
| Gene ID (NCBI) | 54820 |
| RRID | AB_2149877 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NDE1 (NUDE /NUDE1) is a LIS1-interacting protein. A signaling pathway containing NDE1, LIS1, and cytoplasmic dynein is conserved for eukaryotic nuclear migration and has profound impact on neuronal migration during development of the central nervous system. LIS1 and dynein have been shown to be involved in mitosis and NDE1 participates in a subset of dynein function in M phase. NDE1 is specifically phosphorylated in M phase in its serine/threonine phosphorylation motifs, probably by Cdc2 and also Erk1 and Erk2. A fraction of NDEl binds to centrosomes strongly in interphase and is localized to mitotic spindles in early M phase. Phosphorylation of NDE1 also regulates the cell-cycle-dependent distribution, possibly by increasing its dissociation rate at the microtubule-organizing center. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human NDE1.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NDE1 antibody 10233-1-AP | Download protocol |
| IHC protocol for NDE1 antibody 10233-1-AP | Download protocol |
| IF protocol for NDE1 antibody 10233-1-AP | Download protocol |
| IP protocol for NDE1 antibody 10233-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Science A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes. | ||
Nat Cell Biol Nde1-mediated inhibition of ciliogenesis affects cell cycle re-entry.
| ||
Neuron Posterior Neocortex-Specific Regulation of Neuronal Migration by CEP85L Identifies Maternal Centriole-Dependent Activation of CDK5. | ||
Am J Hum Genet The essential role of centrosomal NDE1 in human cerebral cortex neurogenesis. | ||
J Cell Biol Mitotic control of kinetochore-associated dynein and spindle orientation by human Spindly. | ||
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Philip (Verified Customer) (09-04-2019) | Cells fixed in PTEMF for 10min at RT. Blocked in PBS+3% BSA for 30min at RT. Primary at 1/500 for 1hr at RT.
|
FH Aurelie (Verified Customer) (09-02-2019) | lysates (15µg) from human primary Fibroblasts were loaded in 8% SDS-PAGE and immunoblotted using anti-Nde1 ( 1/1500) human primary Fibroblasts were fixed in PFA 4% and stained using anti-Nde1 ( 1/100) over-night at 4°C followed by 1h at RT with a secondary anti-rabbit Alexa (594nM) .
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