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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated NDE1 Polyclonal antibody

NDE1 Polyclonal Antibody for IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-10233

Synonyms

FLJ20101, HOM TES 87, NDE1, NUDE, NUDE1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using CoraLite® Plus 488-conjugated NDE1 Polyclonal anti (CL488-10233)

    IF Staining of human breast cancer using CL488-10233

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite® Plus 488 NDE1 antibody (CL488-10233) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using CoraLite® Plus 488-conjugated NDE1 Polyclonal anti (CL488-10233)

    IF Staining of human breast cancer using CL488-10233

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite® Plus 488 NDE1 antibody (CL488-10233) at dilution of 1:200.

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Tested Applications

Positive IF-P detected inhuman breast cancer tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-10233 targets NDE1 in IF-P applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NDE1 fusion protein Ag0336 相同性解析による交差性が予測される生物種
Full Name nudE nuclear distribution gene E homolog 1 (A. nidulans)
Calculated molecular weight 335 aa, 38 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC001421
Gene symbol NDE1
Gene ID (NCBI) 54820
RRIDAB_2918964
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NDE1 (NUDE /NUDE1) is a LIS1-interacting protein. A signaling pathway containing NDE1, LIS1, and cytoplasmic dynein is conserved for eukaryotic nuclear migration and has profound impact on neuronal migration during development of the central nervous system. LIS1 and dynein have been shown to be involved in mitosis and NDE1 participates in a subset of dynein function in M phase. NDE1 is specifically phosphorylated in M phase in its serine/threonine phosphorylation motifs, probably by Cdc2 and also Erk1 and Erk2. A fraction of NDEl binds to centrosomes strongly in interphase and is localized to mitotic spindles in early M phase. Phosphorylation of NDE1 also regulates the cell-cycle-dependent distribution, possibly by increasing its dissociation rate at the microtubule-organizing center. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human NDE1.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 NDE1 antibody CL488-10233Download protocol
Standard Protocols
Click here to view our Standard Protocols