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  • KD/KO Validated

NDRG1 Polyclonal antibody

NDRG1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 26902-1-AP

Synonyms

CAP43, CMT4D, DRG 1, DRG1, GC4, HMSNL, N myc downstream regulated 1, NDR1, NDRG1, Protein NDRG1, PROXY1, RIT42, RTP, TARG1, TDD5


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Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26902-1-AP targets NDRG1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NDRG1 fusion protein Ag25424 相同性解析による交差性が予測される生物種
Full Name N-myc downstream regulated 1
Calculated molecular weight 43 kDa
Observed molecular weight 43 kDa
GenBank accession numberBC006260
Gene symbol NDRG1
Gene ID (NCBI) 10397
RRIDAB_2880676
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NDRG1 is a member of the N-myc down-regulated gene family which belongs to the alpha/beta hydrolase superfamily. NDRG1 is a highly conserved cytoplasmic protein involved in cell growth arrest and proliferation, cell differentiation, DNA damage response, heavy metal response, the hypoxia response, tumorigenesis, and metastasis. NDRG1 is a potent metastasis suppressor in multiple tumor-types. NDRG1 appears to serve as a putative tumor suppressor and is down-regulated in colorectal, gastric, cervical, ovarian and prostate cancers.

Protocols

Product Specific Protocols
WB protocol for NDRG1 antibody 26902-1-APDownload protocol
IHC protocol for NDRG1 antibody 26902-1-APDownload protocol
IF protocol for NDRG1 antibody 26902-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

mTORC1 stimulates cell growth through SAM synthesis and m6A mRNA-dependent control of protein synthesis.

Authors - Elodie Villa
humanWB

Nucleic Acids Res

TARG1 protects against toxic DNA ADP-ribosylation.

Authors - Callum Tromans-Coia
  • KD Validated
humanWB,IHC

Cell Oncol (Dordr)

LINC01419 promotes cell proliferation and metastasis in hepatocellular carcinoma by enhancing NDRG1 promoter activity.

Authors - Hao Dang
humanWB

Gynecol Oncol

Deferasirox shows inhibition activity against cervical cancer in vitro and in vivo.

Authors - Nan Zhou
humanFC

Mol Biol Rep

Excessive iodine induces thyroid follicular epithelial cells apoptosis by activating HIF-1α-mediated hypoxia pathway in Hashimoto thyroiditis

Authors - Lili Zhang
humanWB,IHC

PeerJ

LINC00844 promotes proliferation and migration of hepatocellular carcinoma by regulating NDRG1 expression.

Authors - Wei Zhou