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Validation Data Gallery

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  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 647-conjugated NDRG1 Polyclonal ant (CL647-26902)

    FC experiment of HeLa using CL647-26902

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 647 Anti-Human NDRG1 (CL647-26902)(red), or 0.8 ug CoraLite® Plus 647-conjugated Rabbit IgG control Rabbit PolyAb (CL647-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated NDRG1 Polyclonal antibody

NDRG1 Polyclonal Antibody for FC (Intra)
Cat No. CL647-26902

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Synonyms

CAP43, CMT4D, DRG 1, DRG1, GC4, HMSNL, N myc downstream regulated 1, NDR1, NDRG1, Protein NDRG1, PROXY1, RIT42, RTP, TARG1, TDD5

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 647
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-26902 targets NDRG1 in FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NDRG1 fusion protein Ag25424 相同性解析による交差性が予測される生物種
Full Name N-myc downstream regulated 1
Calculated molecular weight 43 kDa
Observed molecular weight 43 kDa
GenBank accession numberBC006260
Gene Symbol NDRG1
Gene ID (NCBI) 10397
RRIDAB_2934963
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92597
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NDRG1 is a member of the N-myc down-regulated gene family which belongs to the alpha/beta hydrolase superfamily. NDRG1 is a highly conserved cytoplasmic protein involved in cell growth arrest and proliferation, cell differentiation, DNA damage response, heavy metal response, the hypoxia response, tumorigenesis, and metastasis. NDRG1 is a potent metastasis suppressor in multiple tumor-types. NDRG1 appears to serve as a putative tumor suppressor and is down-regulated in colorectal, gastric, cervical, ovarian and prostate cancers.

Protocols

Product Specific Protocols
FC protocol for CL Plus 647 NDRG1 antibody CL647-26902Download protocol
Standard Protocols
Click here to view our Standard Protocols