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Species Reactivity

Species Reactivity

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NDUFA4L2 Monoclonal antibody, PBS Only

NDUFA4L2 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G1H10

Cat no : 66050-1-PBS

Synonyms

NDUFA4L2, NUOMS

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using NDUFA4L2 Monoclonal antibody (66050-1-Ig)

    WB analysis of HeLa using 66050-1-Ig (same clone as 66050-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66050-1-Ig (NDUFA4L2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66050-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using NDUFA4L2 Monoclonal antibody (66050-1-Ig)

    WB analysis of HeLa using 66050-1-Ig (same clone as 66050-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66050-1-Ig (NDUFA4L2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66050-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using NDUFA4L2 Monoclonal antibody (66050-1-Ig)

    IHC staining of human breast cancer using 66050-1-Ig (same clone as 66050-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66050-1-Ig (NDUFA4L2 Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66050-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using NDUFA4L2 Monoclonal antibody (66050-1-Ig)

    IHC staining of human breast cancer using 66050-1-Ig (same clone as 66050-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66050-1-Ig (NDUFA4L2 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66050-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using NDUFA4L2 Monoclonal antibody (66050-1-Ig)

    IHC staining of human renal cell carcinoma using 66050-1-Ig (same clone as 66050-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 66050-1-Ig (NDUFA4L2 Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66050-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using NDUFA4L2 Monoclonal antibody (66050-1-Ig)

    IHC staining of human renal cell carcinoma using 66050-1-Ig (same clone as 66050-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 66050-1-Ig (NDUFA4L2 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66050-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HUVEC cells using NDUFA4L2 Monoclonal antibody (66050-1-Ig)

    IF Staining of HUVEC using 66050-1-Ig (same clone as 66050-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using NDUFA4L2 antibody (66050-1-Ig, Clone: 1G1H10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66050-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using NDUFA4L2 Monoclonal antibody (66050-1-Ig)

    IF Staining of MCF-7 using 66050-1-Ig (same clone as 66050-1-PBS)

    Immunofluorescent analysis of MCF-7 cells, using NDUFA4L2 antibody 66050-1-Ig at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). This data was developed using the same antibody clone with 66050-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NDUFA4L2 Monoclonal antibody (66050-1-Ig)

    IF Staining of HeLa using 66050-1-Ig (same clone as 66050-1-PBS)

    Immunofluorescent analysis of Hela cells, using NDUFA4L2 antibody 66050-1-Ig at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). This data was developed using the same antibody clone with 66050-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66050-1-PBS targets NDUFA4L2 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen NDUFA4L2 fusion protein Ag9233 相同性解析による交差性が予測される生物種
Full Name NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 4-like 2
Calculated molecular weight 87 aa, 10 kDa
Observed molecular weight 10 kDa
GenBank accession numberBC011910
Gene symbol NDUFA4L2
Gene ID (NCBI) 56901
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

NDUFA4L2, also named as NUOMS, is a HIF-1α target gene that localizes to the mitochondria. It downregulates oxygen consumption and Complex I activity in hypoxia. NDUFA4L2 is involved in hypoxic adaptation by decreasing mitochondrial ROS production.