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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NDUFA4L2 Monoclonal antibody, PBS Only (Capture)

NDUFA4L2 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G1H10

Cat no : 66050-1-PBS

Synonyms

NUOMS, NADH-ubiquinone oxidoreductase MLRQ subunit homolog, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 4-like 2, 1G1H10

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using NDUFA4L2 Monoclonal antibody (66050-1-Ig)

    WB analysis of HeLa using 66050-1-Ig (same clone as 66050-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66050-1-Ig (NDUFA4L2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66050-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using NDUFA4L2 Monoclonal antibody (66050-1-Ig)

    WB analysis of HeLa using 66050-1-Ig (same clone as 66050-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66050-1-Ig (NDUFA4L2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66050-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using NDUFA4L2 Monoclonal antibody (66050-1-Ig)

    IHC staining of human breast cancer using 66050-1-Ig (same clone as 66050-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66050-1-Ig (NDUFA4L2 Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66050-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using NDUFA4L2 Monoclonal antibody (66050-1-Ig)

    IHC staining of human breast cancer using 66050-1-Ig (same clone as 66050-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66050-1-Ig (NDUFA4L2 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66050-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using NDUFA4L2 Monoclonal antibody (66050-1-Ig)

    IHC staining of human renal cell carcinoma using 66050-1-Ig (same clone as 66050-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 66050-1-Ig (NDUFA4L2 Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66050-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using NDUFA4L2 Monoclonal antibody (66050-1-Ig)

    IHC staining of human renal cell carcinoma using 66050-1-Ig (same clone as 66050-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 66050-1-Ig (NDUFA4L2 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66050-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of OS-RC-2 cells using NDUFA4L2 Monoclonal antibody (66050-1-Ig)

    IF Staining of OS-RC-2 using 66050-1-Ig (same clone as 66050-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed OS-RC-2 cells using NDUFA4L2 antibody (66050-1-Ig, Clone: 1G1H10 ) at dilution of 1:1200 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 66050-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HUVEC cells using NDUFA4L2 Monoclonal antibody (66050-1-Ig)

    IF Staining of HUVEC using 66050-1-Ig (same clone as 66050-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using NDUFA4L2 antibody (66050-1-Ig, Clone: 1G1H10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66050-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using NDUFA4L2 Monoclonal antibody (66050-1-Ig)

    IF Staining of MCF-7 using 66050-1-Ig (same clone as 66050-1-PBS)

    Immunofluorescent analysis of MCF-7 cells, using NDUFA4L2 antibody 66050-1-Ig at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). This data was developed using the same antibody clone with 66050-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NDUFA4L2 Monoclonal antibody (66050-1-Ig)

    IF Staining of HeLa using 66050-1-Ig (same clone as 66050-1-PBS)

    Immunofluorescent analysis of Hela cells, using NDUFA4L2 antibody 66050-1-Ig at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). This data was developed using the same antibody clone with 66050-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50529-1

    Cytometric bead array standard curve of MP50529-1

    Cytometric bead array standard curve of MP50529-1, NDUFA4L2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66050-1-PBS. Detection antibody: 66050-2-PBS. Standard:Ag9233. Range: 0.098-100 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66050-1-PBS targets NDUFA4L2 as part of a matched antibody pair:

MP50529-1: 66050-1-PBS capture and 66050-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen NDUFA4L2 fusion protein Ag9233 相同性解析による交差性が予測される生物種
Full Name NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 4-like 2
Calculated molecular weight 87 aa, 10 kDa
Observed molecular weight 10 kDa
GenBank accession numberBC011910
Gene symbol NDUFA4L2
Gene ID (NCBI) 56901
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

NDUFA4L2, also named as NUOMS, is a HIF-1α target gene that localizes to the mitochondria. It downregulates oxygen consumption and Complex I activity in hypoxia. NDUFA4L2 is involved in hypoxic adaptation by decreasing mitochondrial ROS production.