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NDUFA4L2 Monoclonal antibody

NDUFA4L2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66050-1-Ig
Clone No.1G1H10

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

NUOMS, NADH-ubiquinone oxidoreductase MLRQ subunit homolog, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 4-like 2, 1G1H10

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -


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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inHela cells
Positive IHC detected inhuman breast cancer tissue, human renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHUVEC cells, OS-RC-2 cells, MCF-7 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66050-1-Ig targets NDUFA4L2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen NDUFA4L2 fusion protein Ag9233 相同性解析による交差性が予測される生物種
Full Name NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 4-like 2
Calculated molecular weight 87 aa, 10 kDa
Observed molecular weight 10 kDa
GenBank accession numberBC011910
Gene Symbol NDUFA4L2
Gene ID (NCBI) 56901
RRIDAB_11045656
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NRX3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NDUFA4L2, also named as NUOMS, is a HIF-1α target gene that localizes to the mitochondria. It downregulates oxygen consumption and Complex I activity in hypoxia. NDUFA4L2 is involved in hypoxic adaptation by decreasing mitochondrial ROS production.

Protocols

Product Specific Protocols
WB protocol for NDUFA4L2 antibody 66050-1-IgDownload protocol
IHC protocol for NDUFA4L2 antibody 66050-1-IgDownload protocol
IF protocol for NDUFA4L2 antibody 66050-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Elife

Molecular classification and tumor microenvironment characteristics in pheochromocytomas

Authors - Sen Qin
humanWB

Cancer Biol Ther

NDUFA4L2 reduces mitochondrial respiration resulting in defective lysosomal trafficking in clear cell renal cell carcinoma

Authors - Jaclyn M Kubala
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