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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated NDUFA4L2 Monoclonal antibody

NDUFA4L2 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1G1H10

Cat no : CL488-66050

Synonyms

FLJ26118, NDUFA4L2, NUOMS

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CoraLite® Plus 488-conjugated NDUFA4L2 Monoclonal (CL488-66050)

    IF Staining of MCF-7 using CL488-66050

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CL488-66050 (NDUFA4L2 antibody) at dilution of 1:100.

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Tested Applications

Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL488-66050 is NDUFA4L2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen NDUFA4L2 fusion protein Ag9233 相同性解析による交差性が予測される生物種
Full Name NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 4-like 2
Calculated molecular weight 87 aa, 10 kDa
Observed molecular weight 10 kDa
GenBank accession numberBC011910
Gene symbol NDUFA4L2
Gene ID (NCBI) 56901
RRIDAB_2883240
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NDUFA4L2, also named as NUOMS, is a HIF-1α target gene that localizes to the mitochondria. It downregulates oxygen consumption and Complex I activity in hypoxia. NDUFA4L2 is involved in hypoxic adaptation by decreasing mitochondrial ROS production.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 NDUFA4L2 antibody CL488-66050Download protocol
Standard Protocols
Click here to view our Standard Protocols