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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NDUFB7 Monoclonal antibody

NDUFB7 Monoclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1H8C1

Cat no : 68362-1-Ig

Synonyms

Complex I B18, CI-B18, CI B18, Cell adhesion protein SQM1, B18

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NDUFB7 Monoclonal antibody (68362-1-Ig)

    WB analysis using 68362-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68362-1-Ig (NDUFB7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using NDUFB7 Monoclonal antibody (68362-1-Ig)

    IHC staining of mouse heart using 68362-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68362-1-Ig (NDUFB7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using NDUFB7 Monoclonal antibody (68362-1-Ig)

    IHC staining of mouse heart using 68362-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68362-1-Ig (NDUFB7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of HEK-293 cells using NDUFB7 Monoclonal antibody (68362-1-Ig)

    FC experiment of HEK-293 using 68362-1-Ig

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human NDUFB7 (68362-1-Ig, Clone:1H8C1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inLNCaP cells, MCF-7 cells, HeLa cells, HEK-293 cells, PC-3 cell, pig heart tissue, rabbit heart tissue, rat heart tissue, mouse heart tissue
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68362-1-Ig targets NDUFB7 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NDUFB7 fusion protein Ag6720 相同性解析による交差性が予測される生物種
Full Name NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 7, 18kDa
Calculated molecular weight 16 kDa
Observed molecular weight18 kDa
GenBank accession numberBC002595
Gene symbol NDUFB7
Gene ID (NCBI) 4713
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NDUFB7(NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7) is also named as CI-B18 and belongs to the complex I NDUFB7 subunit family. It is an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), which couples the oxidation of NADH to the reduction of ubiquinone and the translocation of protons across the inner membrane. NDUFB7 protein lacks a mitochondrial targeting signal and transmembrane domains. However, it has a Cx(9)C motif that includes an intermembrane space targeting signal.(PMID:21310150).

Protocols

Product Specific Protocols
WB protocol for NDUFB7 antibody 68362-1-IgDownload protocol
IHC protocol for NDUFB7 antibody 68362-1-IgDownload protocol
FC protocol for NDUFB7 antibody 68362-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols