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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NDUFB7 Monoclonal antibody, PBS Only

NDUFB7 Monoclonal Antibody for Indirect ELISA, IHC, WB

Host / Isotype

Mouse / IgG1

Reactivity

rabbit, pig, rat, mouse, human

Applications

Indirect ELISA, IHC, WB

Conjugate

Unconjugated

CloneNo.

1H8C1

Cat no : 68362-1-PBS

Synonyms

Complex I B18, CI-B18, CI B18, Cell adhesion protein SQM1, B18

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NDUFB7 Monoclonal antibody (68362-1-Ig)

    WB analysis using 68362-1-Ig (same clone as 68362-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68362-1-Ig (NDUFB7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68362-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using NDUFB7 Monoclonal antibody (68362-1-Ig)

    IHC staining of mouse heart using 68362-1-Ig (same clone as 68362-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68362-1-Ig (NDUFB7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68362-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using NDUFB7 Monoclonal antibody (68362-1-Ig)

    IHC staining of mouse heart using 68362-1-Ig (same clone as 68362-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68362-1-Ig (NDUFB7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68362-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HEK-293 cells using NDUFB7 Monoclonal antibody (68362-1-Ig)

    FC experiment of HEK-293 using 68362-1-Ig (same clone as 68362-1-PBS)

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human NDUFB7 (68362-1-Ig, Clone:1H8C1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68362-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68362-1-PBS targets NDUFB7 in Indirect ELISA, IHC, WB applications and shows reactivity with rabbit, pig, rat, mouse, human samples.

Tested Reactivity rabbit, pig, rat, mouse, human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NDUFB7 fusion protein Ag6720 相同性解析による交差性が予測される生物種
Full Name NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 7, 18kDa
Calculated molecular weight 16 kDa
Observed molecular weight18 kDa
GenBank accession numberBC002595
Gene symbol NDUFB7
Gene ID (NCBI) 4713
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

NDUFB7(NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7) is also named as CI-B18 and belongs to the complex I NDUFB7 subunit family. It is an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), which couples the oxidation of NADH to the reduction of ubiquinone and the translocation of protons across the inner membrane. NDUFB7 protein lacks a mitochondrial targeting signal and transmembrane domains. However, it has a Cx(9)C motif that includes an intermembrane space targeting signal.(PMID:21310150).