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Species Reactivity
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NDUFB8 Recombinant antibody, PBS Only

NDUFB8 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230510B10

Cat no : 83216-3-PBS

Synonyms

Complex I-ASHI, CI-ASHI, CIASHI, CI ASHI, ASHI

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NDUFB8 Recombinant antibody (83216-3-RR)

    WB analysis using 83216-3-RR (same clone as 83216-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83216-3-RR (NDUFB8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83216-3-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HepG2 cells using NDUFB8 Recombinant antibody (83216-3-RR)

    IP experiment of HepG2 using 83216-3-RR (same clone as 83216-3-PBS)

    IP result of anti-NDUFB8 (IP:83216-3-RR, 4ug; Detection:83216-3-RR 1:3000) with HepG2 cells lysate 1470 ug. This data was developed using the same antibody clone with 83216-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using NDUFB8 Recombinant antibody (83216-3-RR)

    IHC staining of human stomach cancer using 83216-3-RR (same clone as 83216-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83216-3-RR (NDUFB8 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9). This data was developed using the same antibody clone with 83216-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using NDUFB8 Recombinant antibody (83216-3-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 83216-3-RR (same clone as 83216-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83216-3-RR (NDUFB8 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83216-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NDUFB8 Recombinant antibody (83216-3-RR)

    IF Staining of HepG2 using 83216-3-RR (same clone as 83216-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NDUFB8 antibody (83216-3-RR, Clone: 230510B10 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83216-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NDUFB8 Recombinant antibody (83216-3-RR)

    IF Staining of HepG2 using 83216-3-RR (same clone as 83216-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NDUFB8 antibody (83216-3-RR, Clone: 230510B10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83216-3-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using NDUFB8 Recombinant antibody (83216-3-RR)

    FC experiment of HeLa using 83216-3-RR (same clone as 83216-3-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug NDUFB8 Recombinant antibody (83216-3-RR, Clone:230510B10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83216-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83216-3-RR

    Affinity and Kinetic Characterization of 83216-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83216-3-RR against Human NDUFB8 were performed. The affinity constant is 3.26 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83216-3-PBS is NDUFB8 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NDUFB8 fusion protein Ag6495 相同性解析による交差性が予測される生物種
Full Name NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 8, 19kDa
Calculated molecular weight 22 kDa
Observed molecular weight 19-22 kDa
GenBank accession numberBC000466
Gene symbol NDUFB8
Gene ID (NCBI) 4714
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.