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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NDUFB8 Recombinant antibody

NDUFB8 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

CloneNo.

230510B10

Cat no : 83216-3-RR

Synonyms

Complex I-ASHI, CI-ASHI, CIASHI, CI ASHI, ASHI

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NDUFB8 Recombinant antibody (83216-3-RR)

    WB analysis using 83216-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83216-3-RR (NDUFB8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using NDUFB8 Recombinant antibody (83216-3-RR)

    IP experiment of HepG2 using 83216-3-RR

    IP result of anti-NDUFB8 (IP:83216-3-RR, 4ug; Detection:83216-3-RR 1:3000) with HepG2 cells lysate 1470 ug.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using NDUFB8 Recombinant antibody (83216-3-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 83216-3-RR

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83216-3-RR (NDUFB8 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using NDUFB8 Recombinant antibody (83216-3-RR)

    IHC staining of human stomach cancer using 83216-3-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83216-3-RR (NDUFB8 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NDUFB8 Recombinant antibody (83216-3-RR)

    IF Staining of HepG2 using 83216-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NDUFB8 antibody (83216-3-RR, Clone: 230510B10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NDUFB8 Recombinant antibody (83216-3-RR)

    IF Staining of HepG2 using 83216-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NDUFB8 antibody (83216-3-RR, Clone: 230510B10 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using NDUFB8 Recombinant antibody (83216-3-RR)

    FC experiment of HeLa using 83216-3-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug NDUFB8 Recombinant antibody (83216-3-RR, Clone:230510B10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83216-3-RR

    Affinity and Kinetic Characterization of 83216-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83216-3-RR against Human NDUFB8 were performed. The affinity constant is 3.26 nM.

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Tested Applications

Positive WB detected inHepG2 cells, A549 cells, mouse liver tissue, mouse brain tissue, rat brain tissue, HeLa cells, Jurkat cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman stomach cancer tissue, human intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83216-3-RR is NDUFB8 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NDUFB8 fusion protein Ag6495 相同性解析による交差性が予測される生物種
Full Name NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 8, 19kDa
Calculated molecular weight 22 kDa
Observed molecular weight 19-22 kDa
GenBank accession numberBC000466
Gene symbol NDUFB8
Gene ID (NCBI) 4714
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NDUFB8 antibody 83216-3-RRDownload protocol
IHC protocol for NDUFB8 antibody 83216-3-RRDownload protocol
IF protocol for NDUFB8 antibody 83216-3-RRDownload protocol
IP protocol for NDUFB8 antibody 83216-3-RRDownload protocol
FC protocol for NDUFB8 antibody 83216-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols