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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated NDUFS1 Monoclonal antibody

NDUFS1 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3H5E8

Cat no : CL488-68253

Synonyms

EC:7.1.1.2, Complex I-75kD, Complex I 75kD, CI-75kD, CI 75Kd

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated NDUFS1 Monoclonal an (CL488-68253)

    IF Staining of HepG2 using CL488-68253

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 NDUFS1 antibody (CL488-68253, Clone: 3H5E8 ) at dilution of 1:100.

  • Flow cytometry (FC) experiment of Jurkat cells using CoraLite® Plus 488-conjugated NDUFS1 Monoclonal an (CL488-68253)

    FC experiment of Jurkat using CL488-68253

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human NDUFS1 (CL488-68253, Clone:3H5E8) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inJurkat cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68253 targets NDUFS1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NDUFS1 fusion protein Ag3135 相同性解析による交差性が予測される生物種
Full Name NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75kDa (NADH-coenzyme Q reductase)
Calculated molecular weight 727 aa, 79 kDa
Observed molecular weight68-81 kDa
GenBank accession numberBC030833
Gene symbol NDUFS1
Gene ID (NCBI) 4719
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The multisubunit NADH:ubiquinone oxidoreductase (75 kDa subunit, mitochondrial)(NDUFS1) is the first enzyme complex in the electron transport chain of mitochondria. It is also named as Complex I-75kD. By use of chaotropic agents, complex I can be fragmented into 3 different fractions: a flavoprotein fraction, an iron-sulfur protein (IP) fraction, and a hydrophobic protein (HP) fraction. NDUFS1 is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. NDUFS1 has some isoforms with the molecular mass of 68-81 kDa.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 NDUFS1 antibody CL488-68253Download protocol
Standard Protocols
Click here to view our Standard Protocols