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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NEDD1 Recombinant antibody, PBS Only

NEDD1 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240404A7

Cat no : 83443-6-PBS

Synonyms

Protein NEDD1, Neural precursor cell expressed developmentally down-regulated protein 1, NEDD-1, NEDD 1, 240404A7

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NEDD1 Recombinant antibody (83443-6-RR)

    WB analysis using 83443-6-RR (same clone as 83443-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83443-6-RR (NEDD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83443-6-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HEK-293 cells using NEDD1 Recombinant antibody (83443-6-RR)

    FC experiment of HEK-293 using 83443-6-RR (same clone as 83443-6-PBS)

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug Nedd1 Recombinant Antibody (83443-6-RR, Clone:240404A7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83443-6-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A431 cells using NEDD1 Recombinant antibody (83443-6-RR)

    FC experiment of A431 using 83443-6-RR (same clone as 83443-6-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug Nedd1 Recombinant Antibody (83443-6-RR, Clone:240404A7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83443-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83443-6-RR

    Affinity and Kinetic Characterization of 83443-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83443-6-RR against Human NEDD1 were performed. The affinity constant is below 1 pM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83443-6-PBS is NEDD1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NEDD1 fusion protein Ag33603 相同性解析による交差性が予測される生物種
Full Name neural precursor cell expressed, developmentally down-regulated 1
Calculated molecular weight 72 kDa
GenBank accession numberBC027605
Gene symbol NEDD1
Gene ID (NCBI) 121441
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.