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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NEDD1 Recombinant antibody

NEDD1 Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240404A7

Cat no : 83443-6-RR

Synonyms

Protein NEDD1, Neural precursor cell expressed developmentally down-regulated protein 1, NEDD-1, NEDD 1, 240404A7

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NEDD1 Recombinant antibody (83443-6-RR)

    WB analysis using 83443-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83443-6-RR (NEDD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HEK-293 cells using NEDD1 Recombinant antibody (83443-6-RR)

    FC experiment of HEK-293 using 83443-6-RR

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug Nedd1 Recombinant Antibody (83443-6-RR, Clone:240404A7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Flow cytometry (FC) experiment of A431 cells using NEDD1 Recombinant antibody (83443-6-RR)

    FC experiment of A431 using 83443-6-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug Nedd1 Recombinant Antibody (83443-6-RR, Clone:240404A7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83443-6-RR

    Affinity and Kinetic Characterization of 83443-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83443-6-RR against Human NEDD1 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inSH-SY5Y cells, HEK-293 cells, HeLa cells, Jurkat cells, MCF-7 cells
Positive FC (Intra) detected inA431 cells, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83443-6-RR is NEDD1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NEDD1 fusion protein Ag33603 相同性解析による交差性が予測される生物種
Full Name neural precursor cell expressed, developmentally down-regulated 1
Calculated molecular weight 72 kDa
Observed molecular weight71-75 kDa
GenBank accession numberBC027605
Gene symbol NEDD1
Gene ID (NCBI) 121441
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NEDD1 (NEDD1 gamma-tubulin ring complex targeting factor), also known as GCP-WD. It is expected to be located in centrosome; nucleoplasm; and plasma membrane. Required for mitosis progression. Promotes the nucleation of microtubules from the spindle. The molecular weight of NEDD1is 72 kDa.

Protocols

Product Specific Protocols
WB protocol for NEDD1 antibody 83443-6-RRDownload protocol
FC protocol for NEDD1 antibody 83443-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols