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  • Featured Product
  • KD/KO Validated

NEIL3 Polyclonal antibody, PBS Only

NEIL3 Polyclonal Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11621-1-PBS

Synonyms

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HEK-293 cells using NEIL3 Polyclonal antibody (11621-1-AP)

    WB analysis of HEK-293 using 11621-1-AP (same clone as 11621-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11621-1-AP (NEIL3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11621-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using NEIL3 Polyclonal antibody (11621-1-AP)

    WB analysis of HEK-293 using 11621-1-AP (same clone as 11621-1-PBS)

    WB result of NEIL3 antibody (11621-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NEIL3 transfected HEK-293 cells. This data was developed using the same antibody clone with 11621-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using NEIL3 Polyclonal antibody (11621-1-AP)

    WB analysis using 11621-1-AP (same clone as 11621-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11621-1-AP (NEIL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11621-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using NEIL3 Polyclonal antibody (11621-1-AP)

    WB analysis of HEK-293 using 11621-1-AP (same clone as 11621-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11621-1-AP (NEIL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11621-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using NEIL3 Polyclonal antibody (11621-1-AP)

    WB analysis of A431 using 11621-1-AP (same clone as 11621-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 11621-1-AP (NEIL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11621-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse skin tissue using NEIL3 Polyclonal antibody (11621-1-AP)

    WB analysis of mouse skin using 11621-1-AP (same clone as 11621-1-PBS)

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 11621-1-AP (NEIL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11621-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human skin tissue using NEIL3 Polyclonal antibody (11621-1-AP)

    WB analysis of human skin using 11621-1-AP (same clone as 11621-1-PBS)

    human skin tissue were subjected to SDS PAGE followed by western blot with 11621-1-AP (NEIL3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11621-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11621-1-PBS targets NEIL3 in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NEIL3 fusion protein Ag2200 相同性解析による交差性が予測される生物種
Full Name nei endonuclease VIII-like 3 (E. coli)
Calculated molecular weight 605 aa, 68 kDa
Observed molecular weight 62-68 kDa
GenBank accession numberBC025954
Gene symbol NEIL3
Gene ID (NCBI) 55247
RRIDAB_2251229
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

NEIL3 belongs to the FPG family. It is a functional DNA glycosylase in vitro and in vivo. NEIL3 plays a role in repairing FapyG in vivo. (PMID:20185759) NEIL3 has AP lyase activity specific for single-stranded DNA.(PMID:19170771). NEIL3 is specifically expressed in brain areas where neurogenesis takes place during development and that its expression is tightly regulated both temporally and spatially (PMID:19426544). Studies have shown that NEIL3 is recruited to the DSB sites through its GRF zinc finger motifs and plays an important role in MMC/cisplatin-ICL repair by promoting the HR step in FA/BRCA pathway.(PMID: 36351389).