Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis of human peripheral blood leukocyte using 66632-1-Ig
Human peripheral blood leukocyte were subjected to SDS PAGE followed by western blot with 66632-1-Ig (NEK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of rat thymus using 66632-1-Ig
rat thymus tissue were subjected to SDS PAGE followed by western blot with 66632-1-Ig (NEK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of THP-1 using 66632-1-Ig
THP-1 cells were subjected to SDS PAGE followed by western blot with 66632-1-Ig (NEK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 66632-1-Ig
K-562 cells were subjected to SDS PAGE followed by western blot with 66632-1-Ig (NEK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HL-60 using 66632-1-Ig
HL-60 cells were subjected to SDS PAGE followed by western blot with 66632-1-Ig (NEK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 66632-1-Ig
Jurkat cells were subjected to SDS PAGE followed by western blot with 66632-1-Ig (NEK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of RAW 264.7 using 66632-1-Ig
RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66632-1-Ig (NEK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IHC staining of human testis using 66632-1-Ig
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66632-1-Ig (NEK2 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human testis using 66632-1-Ig
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66632-1-Ig (NEK2 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of A431 using 66632-1-Ig
Immunofluorescent analysis of (4% PFA) fixed A431 cells using NEK2 antibody (66632-1-Ig, Clone: 3D7B4 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

NEK2 Monoclonal antibody

NEK2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 66632-1-Ig

Clone No.3D7B4

Publications(2)

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse , Rat and More (2)

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Synonyms

HSPK 21, HsPK21, NEK2, NEK2A, NimA like protein kinase 1, NimA related protein kinase 2, NLK1

Formulation: PBS and Azide

PBS and Azide

PBS Only

Conjugate: Unconjugated

Unconjugated

CoraLite®594

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	Human peripheral blood leukocyte, THP-1 cells, K-562 cells, HL-60 cells, Jurkat cells, RAW 264.7 cells, rat thymus tissue
Positive IHC detected in	human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A431 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunohistochemistry (IHC)	IHC : 1:150-1:600
Immunofluorescence (IF)/ICC	IF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 2 publications below
IF	See 1 publications below
CoIP	See 1 publications below

Product Information

66632-1-Ig targets NEK2 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with Human, Mouse , Rat samples.

Tested Reactivity	Human, Mouse , Rat
Cited Reactivity	human, rat
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	NEK2 fusion protein Ag21476 相同性解析による交差性が予測される生物種
Full Name	NIMA (never in mitosis gene a)-related kinase 2
Calculated molecular weight	52 kDa
Observed molecular weight	48 kDa
GenBank accession number	BC043502
Gene Symbol	NEK2
Gene ID (NCBI)	4751
RRID	AB_2881991
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	P51955
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for NEK2 antibody 66632-1-Ig	Download protocol
IHC protocol for NEK2 antibody 66632-1-Ig	Download protocol
IF protocol for NEK2 antibody 66632-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
rat	WB	Toxicol Lett Oxcarbazepine induces mitotic catastrophe and apoptosis in NRK-52E proximal tubular cells. Authors - Momoka Ota View Article
human	WB,IF,CoIP	Cancer Cell Int NEK2 contributes to radioresistance in esophageal squamous cell carcinoma by inducing protective autophagy via regulating TRIM21 Authors - Dong Guo KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of human peripheral blood leukocyte using 66632-1-Ig

WB analysis of rat thymus using 66632-1-Ig

WB analysis of THP-1 using 66632-1-Ig

WB analysis of K-562 using 66632-1-Ig

WB analysis of HL-60 using 66632-1-Ig

WB analysis of Jurkat using 66632-1-Ig

WB analysis of RAW 264.7 using 66632-1-Ig

IHC staining of human testis using 66632-1-Ig