CoraLite®594-conjugated NEK2 Monoclonal antibody
NEK2 Monoclonal Antibody for FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat
Applications
FC (Intra)
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
3D7B4
Cat no : CL594-66632
Synonyms
Validation Data Gallery
Tested Applications
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL594-66632 targets NEK2 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | NEK2 fusion protein Ag21476 相同性解析による交差性が予測される生物種 |
| Full Name | NIMA (never in mitosis gene a)-related kinase 2 |
| Calculated molecular weight | 52 kDa |
| Observed molecular weight | 48 kDa |
| GenBank accession number | BC043502 |
| Gene symbol | NEK2 |
| Gene ID (NCBI) | 4751 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NEK2, also named as HSPK 21, belongs to the NEK family. NEK2 is well recognized as a multifunctional protein with roles in cell cycle regulation, such as centrosome duplication and separation, microtubule stabilization, kinetochore attachment and spindle assembly checkpoint. NEK2 is highly expressed in several cancer types, such as cholangiocarcinoma, breast, colorectal, and pancreatic cancer. NEK2 in mammals has three splice variants: NEK2A, NEK2B, and NEK2C. NEK2A is evenly distributed within the nuclei and cytoplasm, while NEK2B is mainly distributed in the cytoplasm, and NEK2C is mainly distributed in the nuclear region. As NEK2A, NEK2B and NEK2C exhibit overlapping or identical substrate usage, these variants are collectively referred to here as NEK2 (PMID:31092416).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL594 NEK2 antibody CL594-66632 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
