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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NEK9 Polyclonal antibody (11192-1-AP)

    WB analysis using 11192-1-AP (same clone as 11192-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11192-1-AP (NEK9 antibody) at dilution of 1:4500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11192-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using NEK9 Polyclonal antibody (11192-1-AP)

    WB analysis of HeLa using 11192-1-AP (same clone as 11192-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 11192-1-AP (NEK9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11192-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using NEK9 Polyclonal antibody (11192-1-AP)

    WB analysis of human placenta using 11192-1-AP (same clone as 11192-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 11192-1-AP (NEK9 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11192-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using NEK9 Polyclonal antibody (11192-1-AP)

    IP experiment of HeLa using 11192-1-AP (same clone as 11192-1-PBS)

    IP result of anti-NEK9 (IP:11192-1-AP, 4ug; Detection:11192-1-AP 1:1000) with HeLa cells lysate 1880 ug. This data was developed using the same antibody clone with 11192-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using NEK9 Polyclonal antibody (11192-1-AP)

    IHC staining of human lymphoma using 11192-1-AP (same clone as 11192-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11192-1-AP (NEK9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11192-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using NEK9 Polyclonal antibody (11192-1-AP)

    IHC staining of human lymphoma using 11192-1-AP (same clone as 11192-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11192-1-AP (NEK9 antibody) at dilution of 1:200 (under 0x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11192-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NEK9 Polyclonal antibody (11192-1-AP)

    IF Staining of HepG2 using 11192-1-AP (same clone as 11192-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using NEK9 antibody 11192-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 11192-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NEK9 Polyclonal antibody (11192-1-AP)

    IF Staining of HepG2 using 11192-1-AP (same clone as 11192-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NEK9 antibody (11192-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11192-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using NEK9 Polyclonal antibody (11192-1-AP)

    FC experiment of HeLa using 11192-1-AP (same clone as 11192-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human NEK9 (11192-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug x. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 11192-1-PBS in a different storage buffer formulation.

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NEK9 Polyclonal antibody, PBS Only

NEK9 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 11192-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11192-1-PBS targets NEK9 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NEK9 fusion protein Ag1678 相同性解析による交差性が予測される生物種
Full Name NIMA (never in mitosis gene a)- related kinase 9
Calculated molecular weight 107 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC009336
Gene Symbol NEK9
Gene ID (NCBI) 91754
RRIDAB_2150800
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TD19
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

NEK9 is also named as KIAA1995, NEK8, NERCC and belongs to the NEK Ser/Thr protein kinase family. It is originally identified as αβ-casein kinase that associates with a putative substrate Bicd2. It is also independently identified as a Nek6- and Ran GTPase-binding protein under a different name, Nercc1(PMID:14660563). Nek9, together with the highly similar (80% identical) Nek6 and Nek7, form a signaling module that is activated during mitosis and involved in the regulation of the mitotic spindle. The endogenous Nek9 (with predicted molecular mass of 120 kDa) has an apparent molecular mass of 600 kDa, also compatible with a tetramer(PMID:21454704).