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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NELFB Recombinant antibody, PBS Only (Capture)

NELFB Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241434F12

Cat no : 84135-4-PBS

Synonyms

NELF B, Negative elongation factor B, KIAA1182, Cofactor of BRCA1, COBRA1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NELFB Recombinant antibody (84135-4-RR)

    WB analysis using 84135-4-RR (same clone as 84135-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84135-4-RR (NELF-B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84135-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using NELFB Recombinant antibody (84135-4-RR)

    IF Staining of A431 using 84135-4-RR (same clone as 84135-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using NELF-B antibody (84135-4-RR, Clone: 241434F12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84135-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A431 cells using NELFB Recombinant antibody (84135-4-RR)

    FC experiment of A431 using 84135-4-RR (same clone as 84135-4-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug NELF-B Recombinant antibody (84135-4-RR, Clone:241434F12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84135-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of BxPC-3 cells using NELFB Recombinant antibody (84135-4-RR)

    FC experiment of BxPC-3 using 84135-4-RR (same clone as 84135-4-PBS)

    1x10^6 BxPC-3 cells were intracellularly stained with 0.25 ug NELF-B Recombinant antibody (84135-4-RR, Clone:241434F12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84135-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01055-1

    Cytometric bead array standard curve of MP01055-1

    Cytometric bead array standard curve of MP01055-1, NELF-B Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84135-4-PBS. Detection antibody: 84135-1-PBS. Standard: Ag9599. Range: 0.781-100 ng/mL.

  • Cytometric bead array standard curve of MP01055-3

    Cytometric bead array standard curve of MP01055-3

    Cytometric bead array standard curve of MP01055-3, NELF-B Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84135-4-PBS. Detection antibody: 84135-3-PBS. Standard: Ag9599. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 84135-4-RR

    Affinity and Kinetic Characterization of 84135-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84135-4-RR against Human NELF-B were performed. The affinity constant is 0.199 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84135-4-PBS targets NELFB as part of a matched antibody pair:

MP01055-1: 84135-4-PBS capture and 84135-1-PBS detection (validated in Cytometric bead array)

MP01055-3: 84135-4-PBS capture and 84135-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NELFB fusion protein Ag9599 相同性解析による交差性が予測される生物種
Full Name cofactor of BRCA1
Calculated molecular weight580aa,66 kDa; 475aa,54 kDa
Observed molecular weight 66 kDa
GenBank accession numberBC011892
Gene symbol NELFB
Gene ID (NCBI) 25920
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Cofactor of BRCA1 (COBRA1) was first identified as a BRCA1-interacting protein and subsequently found to be the B subunit of the negative elongation factor complex (NELF-B). Cobra1 is essential for early embryogenesis (PMID: 19340312). The MW of this protein is 66 kDa, and this antibody specially recognises the 66 kDa protein.