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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NELFB Recombinant antibody

NELFB Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241434F12

Cat no : 84135-4-RR

Synonyms

NELF B, Negative elongation factor B, KIAA1182, Cofactor of BRCA1, COBRA1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NELF-B Recombinant antibody (84135-4-RR)

    WB analysis using 84135-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84135-4-RR (NELF-B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using NELF-B Recombinant antibody (84135-4-RR)

    IF Staining of A431 using 84135-4-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using NELF-B antibody (84135-4-RR, Clone: 241434F12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of A431 cells using NELF-B Recombinant antibody (84135-4-RR)

    FC experiment of A431 using 84135-4-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug NELF-B Recombinant antibody (84135-4-RR, Clone:241434F12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Flow cytometry (FC) experiment of BxPC-3 cells using NELF-B Recombinant antibody (84135-4-RR)

    FC experiment of BxPC-3 using 84135-4-RR

    1x10^6 BxPC-3 cells were intracellularly stained with 0.25 ug NELF-B Recombinant antibody (84135-4-RR, Clone:241434F12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 84135-4-RR

    Affinity and Kinetic Characterization of 84135-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84135-4-RR against Human NELF-B were performed. The affinity constant is 0.199 nM.

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, MCF-7 cells, BxPC-3 cells, A431 cells
Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inA431 cells, BxPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84135-4-RR is NELFB Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NELFB fusion protein Ag9599 相同性解析による交差性が予測される生物種
Full Name cofactor of BRCA1
Calculated molecular weight580aa,66 kDa; 475aa,54 kDa
Observed molecular weight 66 kDa
GenBank accession numberBC011892
Gene symbol NELFB
Gene ID (NCBI) 25920
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cofactor of BRCA1 (COBRA1) was first identified as a BRCA1-interacting protein and subsequently found to be the B subunit of the negative elongation factor complex (NELF-B). Cobra1 is essential for early embryogenesis (PMID: 19340312). The MW of this protein is 66 kDa, and this antibody specially recognises the 66 kDa protein.

Protocols

Product Specific Protocols
WB protocol for NELFB antibody 84135-4-RRDownload protocol
IF protocol for NELFB antibody 84135-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols