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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated NET1 Polyclonal antibody

NET1 Polyclonal Antibody for IF-P, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

IF-P, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-28180

Synonyms

ARHGEF8, NET1, NET1A, Proto oncogene p65 Net1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite® Plus 488-conjugated NET1 Polyclonal anti (CL488-28180)

    IF Staining of mouse brain using CL488-28180

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CoraLite® Plus 488 NET1 antibody (CL488-28180) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite® Plus 488-conjugated NET1 Polyclonal anti (CL488-28180)

    IF Staining of mouse brain using CL488-28180

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CoraLite® Plus 488 NET1 antibody (CL488-28180) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of Jurkat cells using CoraLite® Plus 488-conjugated NET1 Polyclonal anti (CL488-28180)

    FC experiment of Jurkat using CL488-28180

    1x10^6 Jurkat cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human NET1 (CL488-28180)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive IF-P detected inmouse brain tissue
Positive FC (Intra) detected inJurkat cells
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-28180 targets NET1 in IF-P, FC (Intra) applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NET1 fusion protein Ag28142 相同性解析による交差性が予測される生物種
Full Name neuroepithelial cell transforming 1
Calculated molecular weight 596 aa, 68 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC010285
Gene symbol NET1
Gene ID (NCBI) 10276
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 NET1 antibody CL488-28180Download protocol
FC protocol for CL Plus 488 NET1 antibody CL488-28180Download protocol
Standard Protocols
Click here to view our Standard Protocols