• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated NEU1 Monoclonal antibody

NEU1 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Pig

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1F6D1

Cat no : CL488-67032

Synonyms

Acetylneuraminyl hydrolase, FLJ93471, G9 sialidase, Lysosomal sialidase, N acetyl alpha neuraminidase 1, NANH, NEU, NEU1, NEURAMINIDASE 1, SIAL1, Sialidase 1

Validation Data Gallery

Filter:
  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using CoraLite® Plus 488-conjugated NEU1 Monoclonal anti (CL488-67032)

    IF Staining of U-251 using CL488-67032

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using CoraLite® Plus 488 NEU1 antibody (CL488-67032, Clone: 1F6D1 ) at dilution of 1:200.

☆大好評トライアルサイズ ¥20,000～！ >>> 一次抗体特別価格キャンペーン開催中！

Tested Applications

Positive IF/ICC detected inU-251 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67032 targets NEU1 in IF/ICC applications and shows reactivity with Human, Rat, Pig samples.

Tested Reactivity Human, Rat, Pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NEU1 fusion protein Ag27945 相同性解析による交差性が予測される生物種
Full Name sialidase 1 (lysosomal sialidase)
Calculated molecular weight 45 kDa
Observed molecular weight 45-48 kDa
GenBank accession numberBC011900
Gene symbol NEU1
Gene ID (NCBI) 4758
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NEU1 (lysosomal sialidase) encodes a kind of lysosomal enzyme that could cleave terminal silic acids from glycoproteins or glycolipids to regulate various biological processes by conformational change (PMID:28130415). In the lysosome, NEU1 is one member of a heterotrimeric complex, the others are beta-galactosidase and cathepsin A. The NEU1 is widely expressed in mammalian tissues and involved in lysosomal storage disorder sialidosis, autoimmune diseases and the malignancy and metastasis of cancer cells (PMID:19075514). NUE1 also expresses on the plasma membrane where it modulates several signalling molecules about inflamation, exocytosis, phagocytosis, cell adhesion and proliferation (PMID:21928149). Addition, it is reported that mutation in NEU1 of human could lead to sialidosis (PMID:14517945).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 NEU1 antibody CL488-67032Download protocol
Standard Protocols
Click here to view our Standard Protocols