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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated NF-M Monoclonal antibody

NF-M Monoclonal Antibody for IF-P, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF-P, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2E3B12

Cat no : CL594-66396

Synonyms

NEFM, NF M, neurofilament, NEF3, 2E3B12

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite®594-conjugated NF-M Monoclonal antibody (CL594-66396)

    IF Staining of mouse brain using CL594-66396

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CoraLite®594 NF-M antibody (CL594-66396, Clone: 2E3B12 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of PC-12 cells using CoraLite®594-conjugated NF-M Monoclonal antibody (CL594-66396)

    FC experiment of PC-12 using CL594-66396

    1x10^6 PC-12 cells were intracellularly stained with 0.8 ug CoraLite®594-conjugated NF-M Monoclonal antibody (CL594-66396, Clone:2E3B12)(red), or 0.8 ug CoraLite®594 Mouse IgG1 Isotype Control (MOPC-21) (CL594-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF-P detected inmouse brain tissue
Positive FC (Intra) detected inPC-12 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66396 targets NF-M in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NF-M fusion protein Ag22709 相同性解析による交差性が予測される生物種
Full Name neurofilament, medium polypeptide
Calculated molecular weight 102 kDa
Observed molecular weight 140 kDa
GenBank accession numberBC002421
Gene symbol NF-M
Gene ID (NCBI) 4741
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NEFM, also named as NEF3 and NFM, belongs to the intermediate filament family. Neurofilaments are the 10nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE: NF-L, 65-68 kDa; NF-M,140-160 kDa and NF-H, 200-220 kDa.

Protocols

Product Specific Protocols
IF protocol for CL594 NF-M antibody CL594-66396Download protocol
Standard Protocols
Click here to view our Standard Protocols