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NF45 Monoclonal antibody

NF45 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 67685-1-Ig
Clone No.1A3A2

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

ILF2, NF45, PRO3063

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inMCF-7 cells, HeLa cells, A431 cells, HepG2 cells, MOLT-4 cells, Jurkat cells, HSC-T6 cells, PC-12 cells, Sp2/0 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67685-1-Ig targets NF45 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen NF45 fusion protein Ag30388 相同性解析による交差性が予測される生物種
Full Name interleukin enhancer binding factor 2, 45kDa
Calculated molecular weight 43 kDa
Observed molecular weight40 kDa
GenBank accession numberBC000382
Gene Symbol ILF2
Gene ID (NCBI) 3608
RRIDAB_2882878
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ12905
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Interleukin-2 enhancer binding factor 2 (ILF2), also named as nuclear factor 45 (NF45), plays important roles in regulating interleukin-2 expression in mammals. It forms a heterodimeric complex with ILF3, which may regulate transcription of the IL2 gene during T-cell activation. It can also promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA [PMID:11739746,17129403].

Protocols

Product Specific Protocols
WB protocol for NF45 antibody 67685-1-IgDownload protocol
IHC protocol for NF45 antibody 67685-1-IgDownload protocol
IF protocol for NF45 antibody 67685-1-IgDownload protocol
IP protocol for NF45 antibody 67685-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IF,CoIP

Cell Rep

Virus-induced lncRNA-BTX allows viral replication by regulating intracellular translocation of DHX9 and ILF3 to induce innate escape

Authors - Yang Cao
humanWB,CoIP

J Exp Clin Cancer Res

LINC00571 drives tricarboxylic acid cycle metabolism in triple-negative breast cancer through HNRNPK/ILF2/IDH2 axis

Authors - Zihan Xi
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