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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NF45 Monoclonal antibody (67685-1-Ig)

    WB analysis using 67685-1-Ig (same clone as 67685-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67685-1-Ig (NF45 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67685-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using NF45 Monoclonal antibody (67685-1-Ig)

    IP experiment of HeLa using 67685-1-Ig (same clone as 67685-1-PBS)

    IP result of anti-NF45 (IP:67685-1-Ig, 4ug; Detection:67685-1-Ig 1:3000) with HeLa cells lysate 1320 ug. This data was developed using the same antibody clone with 67685-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using NF45 Monoclonal antibody (67685-1-Ig)

    IHC staining of human colon cancer using 67685-1-Ig (same clone as 67685-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67685-1-Ig (NF45 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67685-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using NF45 Monoclonal antibody (67685-1-Ig)

    IHC staining of human breast cancer using 67685-1-Ig (same clone as 67685-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67685-1-Ig (NF45 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67685-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using NF45 Monoclonal antibody (67685-1-Ig)

    IHC staining of human colon cancer using 67685-1-Ig (same clone as 67685-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67685-1-Ig (NF45 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67685-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using NF45 Monoclonal antibody (67685-1-Ig)

    IHC staining of human breast cancer using 67685-1-Ig (same clone as 67685-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67685-1-Ig (NF45 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67685-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NF45 Monoclonal antibody (67685-1-Ig)

    IF Staining of HepG2 using 67685-1-Ig (same clone as 67685-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NF45 antibody (67685-1-Ig, Clone: 1A3A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67685-1-PBS in a different storage buffer formulation.

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NF45 Monoclonal antibody, PBS Only

NF45 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 67685-1-PBS
Clone No.1A3A2

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

ILF2, NF45, PRO3063

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67685-1-PBS targets NF45 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen NF45 fusion protein Ag30388 相同性解析による交差性が予測される生物種
Full Name interleukin enhancer binding factor 2, 45kDa
Calculated molecular weight 43 kDa
Observed molecular weight40 kDa
GenBank accession numberBC000382
Gene Symbol ILF2
Gene ID (NCBI) 3608
RRIDAB_2882878
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ12905
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Interleukin-2 enhancer binding factor 2 (ILF2), also named as nuclear factor 45 (NF45), plays important roles in regulating interleukin-2 expression in mammals. It forms a heterodimeric complex with ILF3, which may regulate transcription of the IL2 gene during T-cell activation. It can also promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA [PMID:11739746,17129403].