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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated NFATC3 Monoclonal an (CL488-66924)

    IF Staining of HepG2 using CL488-66924

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 NFATC3 antibody (CL488-66924, Clone: 1C1H8 ) at dilution of 1:200, CL594-Phalloidin (red).

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CoraLite® Plus 488-conjugated NFATC3 Monoclonal antibody

NFATC3 Monoclonal Antibody for IF/ICC
Cat No. CL488-66924
Clone No.1C1H8

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC

Synonyms

NF-AT4c, NF-AT4, NFAT4, NF ATc3, NF AT4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  {{ptg:cur_Conjugation}}
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66924 targets NFATC3 in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NFATC3 fusion protein Ag27306 相同性解析による交差性が予測される生物種
Full Name nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3
Calculated molecular weight 116 kDa
Observed molecular weight 160 kDa
GenBank accession numberBC001050
Gene Symbol NFATC3
Gene ID (NCBI) 4775
RRIDAB_2934505
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ12968
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NFAT (nuclear factors of activated T cells) proteins are a family of transcription factors originally identified as mediators of activation of cytokine genes in response to antigenic stimulation of T cells. NFAT proteins also play varied roles in cells outside of the immune system [PMID:11877454]. NFATc3 has specifically been implicated in vasculature development, regulation of smooth muscle contractile phenotype, and modulation of vascular smooth muscle contractility [PMID:11439183,17148444]. The calculated molecular weight of NFATC3 is 115 kDa, but the post-modified protein is about 130-170 kDa (PMID: 15728531, PMID: 15857835)

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 NFATC3 antibody CL488-66924Download protocol
Standard Protocols
Click here to view our Standard Protocols