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NFIL3 Monoclonal antibody

NFIL3 Monoclonal Antibody for WB, FC (Intra), ELISA
Cat No. 66368-1-Ig
Clone No.2F4B4

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, FC (Intra), ELISA

Interleukin-3-binding protein 1, Interleukin-3 promoter transcriptional activator, IL3BP1, E4BP4, E4 promoter-binding protein 4

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inhuman skeletal muscle tissue
Positive FC (Intra) detected inNK92, NK92 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66368-1-Ig targets NFIL3 in WB, FC (Intra), ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NFIL3 fusion protein Ag2380 相同性解析による交差性が予測される生物種
Full Name nuclear factor, interleukin 3 regulated
Calculated molecular weight 462 aa, 51 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC008197
Gene Symbol NFIL3
Gene ID (NCBI) 4783
RRIDAB_2881748
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ16649
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NFIL3, also named E4BP4 and IL3BP1, belongs to the bZIP family and NFIL3 subfamily. It represses transcription from promoters with activating transcription factor (ATF) sites. NFIL3 activates transcription from the interleukin-3 promoter in T-cells. It competes for the same consensus-binding site with PAR DNA-binding factors (DBP, HLF and TEF). It is a component of the circadian clock that acts as a negative regulator for the circadian expression of PER2 oscillation in the cell-autonomous core clock. NFIL3 protects pro-B cells from programmed cell death. It is a key regulator of TH2 responses(PMID:21499227).

Protocols

Product Specific Protocols
WB protocol for NFIL3 antibody 66368-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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