Validation Data Gallery
Tested Applications
| Positive WB detected in | human skeletal muscle tissue |
| Positive FC (Intra) detected in | NK92, NK92 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66368-1-Ig targets NFIL3 in WB, FC (Intra), ELISA applications and shows reactivity with human, pig samples.
| Tested Reactivity | human, pig |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | NFIL3 fusion protein Ag2380 相同性解析による交差性が予測される生物種 |
| Full Name | nuclear factor, interleukin 3 regulated |
| Calculated molecular weight | 462 aa, 51 kDa |
| Observed molecular weight | 50-55 kDa |
| GenBank accession number | BC008197 |
| Gene Symbol | NFIL3 |
| Gene ID (NCBI) | 4783 |
| RRID | AB_2881748 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q16649 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NFIL3, also named E4BP4 and IL3BP1, belongs to the bZIP family and NFIL3 subfamily. It represses transcription from promoters with activating transcription factor (ATF) sites. NFIL3 activates transcription from the interleukin-3 promoter in T-cells. It competes for the same consensus-binding site with PAR DNA-binding factors (DBP, HLF and TEF). It is a component of the circadian clock that acts as a negative regulator for the circadian expression of PER2 oscillation in the cell-autonomous core clock. NFIL3 protects pro-B cells from programmed cell death. It is a key regulator of TH2 responses(PMID:21499227).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NFIL3 antibody 66368-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |