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Validation Data Gallery

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  • Western Blot (WB) analysis of human skeletal muscle tissue using NFIL3 Monoclonal antibody (66368-1-Ig)

    WB analysis of human skeletal muscle using 66368-1-Ig (same clone as 66368-1-PBS)

    Western blot analysis of NFIL3 in various cell lines and tissues using Proteintech antibody 66368-1-Ig at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66368-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of NK92 using NFIL3 Monoclonal antibody (66368-1-Ig)

    FC experiment of NK92 using 66368-1-Ig (same clone as 66368-1-PBS)

    1X10^6 NK92 were intracellularly stained with 0.4 ug Anti-Human NFIL3 (66368-1-Ig, Clone:2F4B4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug x. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66368-1-PBS in a different storage buffer formulation.

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NFIL3 Monoclonal antibody, PBS Only

NFIL3 Monoclonal Antibody for ELISA, WB
Cat No. 66368-1-PBS
Clone No.2F4B4

Host / Isotype

Mouse / IgG1

Reactivity

pig, human

Applications

ELISA, WB

Synonyms

Interleukin-3-binding protein 1, Interleukin-3 promoter transcriptional activator, IL3BP1, E4BP4, E4 promoter-binding protein 4

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

66368-1-Ig

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66368-1-PBS targets NFIL3 in ELISA, WB applications and shows reactivity with pig, human samples.

Tested Reactivity pig, human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NFIL3 fusion protein Ag2380 相同性解析による交差性が予測される生物種
Full Name nuclear factor, interleukin 3 regulated
Calculated molecular weight 462 aa, 51 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC008197
Gene Symbol NFIL3
Gene ID (NCBI) 4783
RRIDAB_2881748
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ16649
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

NFIL3, also named E4BP4 and IL3BP1, belongs to the bZIP family and NFIL3 subfamily. It represses transcription from promoters with activating transcription factor (ATF) sites. NFIL3 activates transcription from the interleukin-3 promoter in T-cells. It competes for the same consensus-binding site with PAR DNA-binding factors (DBP, HLF and TEF). It is a component of the circadian clock that acts as a negative regulator for the circadian expression of PER2 oscillation in the cell-autonomous core clock. NFIL3 protects pro-B cells from programmed cell death. It is a key regulator of TH2 responses(PMID:21499227).