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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated NFX1 Recombinant ant (CL488-82924)

    IF Staining of HeLa using CL488-82924

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 NFX1 antibody (CL488-82924, Clone: 230211D9 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of PC-3 cells using CoraLite® Plus 488-conjugated NFX1 Recombinant ant (CL488-82924)

    FC experiment of PC-3 using CL488-82924

    1x10^6 PC-3 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Nfx1 Recombinant Antibody (CL488-82924, Clone:230211D9)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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CoraLite® Plus 488-conjugated NFX1 Recombinant antibody

NFX1 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra)
Cat No. CL488-82924
Clone No.230211D9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Synonyms

Transcriptional repressor NF-X1, Nuclear transcription factor, X box-binding protein 1, NFX2, EC:2.3.2.-

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inPC-3 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-82924 targets NFX1 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NFX1 fusion protein Ag9448 相同性解析による交差性が予測される生物種
Full Name nuclear transcription factor, X-box binding 1
Calculated molecular weight 1120 aa, 124 kDa
GenBank accession numberBC012151
Gene Symbol NFX1
Gene ID (NCBI) 4799
RRIDAB_3673118
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ12986
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 NFX1 antibody CL488-82924Download protocol
Standard Protocols
Click here to view our Standard Protocols