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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NHE1 Polyclonal antibody (29761-1-AP)

    WB analysis using 29761-1-AP (same clone as 29761-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29761-1-AP (NHE1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29761-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using NHE1 Polyclonal antibody (29761-1-AP)

    WB analysis using 29761-1-AP (same clone as 29761-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29761-1-AP (NHE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29761-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using NHE1 Polyclonal antibody (29761-1-AP)

    IHC staining of human stomach using 29761-1-AP (same clone as 29761-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 29761-1-AP (NHE1 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 29761-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using NHE1 Polyclonal antibody (29761-1-AP)

    IHC staining of human stomach using 29761-1-AP (same clone as 29761-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 29761-1-AP (NHE1 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 29761-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NHE1 Polyclonal antibody (29761-1-AP)

    IF Staining of HeLa using 29761-1-AP (same clone as 29761-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using NHE1 antibody (29761-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29761-1-PBS in a different storage buffer formulation.

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NHE1 Polyclonal antibody, PBS Only

NHE1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 29761-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

29761-1-PBS targets NHE1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NHE1 fusion protein Ag31192 相同性解析による交差性が予測される生物種
Full Name solute carrier family 9 (sodium/hydrogen exchanger), member 1
Calculated molecular weight 91 kDa
Observed molecular weight90-100 kDa
GenBank accession numberNM_003047
Gene Symbol NHE1
Gene ID (NCBI) 6548
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP19634
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

NHE1, also named as SLC9A1, encodes an Na+/H+ antiporter that is a membrane-bound enzyme involved in regulating pH of vertebrate cells. NHE1 regulates a number of cell behaviors, including adhesion, shape determination, migration, and proliferation (PMID:11807182). It is inhibited by the non-specific diuretic drug amiloride and activated by growth factors, mitogens, neurotransmitters, tumor promoters, and others (PMID: 2846238). NHE1 is expressed in heart where it contributes to cardiomyocyte pH homeostasis (PMID: 12832126, PMID: 23709596).