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- KD/KO Validated
NHE1 Monoclonal antibody
NHE1 Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, Rat and More (2)
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
3D9F3
Cat no : 67363-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, NCI-H1299 cells, HEK-293 cells, LNCaP cells, Jurkat cells, HSC-T6 cells, PC-12 cells |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IHC | See 2 publications below |
Product Information
67363-1-Ig targets NHE1 in WB, IHC, ELISA applications and shows reactivity with Human, Rat samples.
| Tested Reactivity | Human, Rat |
| Cited Reactivity | human, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | NHE1 fusion protein Ag14329 相同性解析による交差性が予測される生物種 |
| Full Name | solute carrier family 9 (sodium/hydrogen exchanger), member 1 |
| Calculated molecular weight | 815 aa, 91 kDa |
| Observed molecular weight | 110 kDa |
| GenBank accession number | BC012121 |
| Gene symbol | NHE1 |
| Gene ID (NCBI) | 6548 |
| RRID | AB_2882615 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NHE1, also named as SLC9A1, is involved in pH regulation that can eliminate acids generated by active metabolism and counter adverse environmental conditions. NHE1 plays an important role in signal transduction. It has been shown that NHE1 participates in the development and progression of human breast cancer, gastric cancer and others. NHE1 has some isoforms with 91 kDa , 110 kDa (phosphoglycoprotein) and 210 kDa (dimeric protein). (PMID:28098891, 27751915, 8300588)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NHE1 antibody 67363-1-Ig | Download protocol |
| IHC protocol for NHE1 antibody 67363-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Acta Pharmacol Sin Direct cardio-protection of Dapagliflozin against obesity-related cardiomyopathy via NHE1/MAPK signaling.
| ||
Reproduction An abnormal LPA/LPAR1-NHE1 axis leads to the autophagy deficiency of trophoblast cells in recurrent spontaneous abortion | ||
Acta Biochim Biophys Sin (Shanghai) Puerarin inhibits NHE1 activity by interfering with the p38 pathway and attenuates mitochondrial damage induced by myocardial calcium overload in heart failure rats |
