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  • Featured Product
  • KD/KO Validated

NHE1 Monoclonal antibody, PBS Only

NHE1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3D9F3

Cat no : 67363-1-PBS

Synonyms

APNH, APNH1, FLJ42224, Na(+)/H(+) exchanger 1, NHE 1, NHE1, SLC9A1, Sodium/hydrogen exchanger 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NHE1 Monoclonal antibody (67363-1-Ig)

    WB analysis using 67363-1-Ig (same clone as 67363-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67363-1-Ig (NHE1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 67363-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using NHE1 Monoclonal antibody (67363-1-Ig)

    IHC staining of human liver using 67363-1-Ig (same clone as 67363-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67363-1-Ig (NHE1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67363-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using NHE1 Monoclonal antibody (67363-1-Ig)

    IHC staining of human liver using 67363-1-Ig (same clone as 67363-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67363-1-Ig (NHE1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67363-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67363-1-PBS targets NHE1 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Rat samples.

Tested Reactivity Human, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NHE1 fusion protein Ag14329 相同性解析による交差性が予測される生物種
Full Name solute carrier family 9 (sodium/hydrogen exchanger), member 1
Calculated molecular weight 815 aa, 91 kDa
Observed molecular weight 110 kDa
GenBank accession numberBC012121
Gene symbol NHE1
Gene ID (NCBI) 6548
RRIDAB_2882615
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

NHE1, also named as SLC9A1, is involved in pH regulation that can eliminate acids generated by active metabolism and counter adverse environmental conditions. NHE1 plays an important role in signal transduction. It has been shown that NHE1 participates in the development and progression of human breast cancer, gastric cancer and others. NHE1 has some isoforms with 91 kDa , 110 kDa (phosphoglycoprotein) and 210 kDa (dimeric protein). (PMID:28098891, 27751915, 8300588)