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Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using NHEDC2 Polyclonal antibody (24065-1-AP)

    WB analysis of human plasma using 24065-1-AP (same clone as 24065-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 24065-1-AP (NHEDC2 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 24065-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using NHEDC2 Polyclonal antibody (24065-1-AP)

    IHC staining of human brain using 24065-1-AP (same clone as 24065-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24065-1-AP (NHEDC2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24065-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using NHEDC2 Polyclonal antibody (24065-1-AP)

    IHC staining of human brain using 24065-1-AP (same clone as 24065-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24065-1-AP (NHEDC2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24065-1-PBS in a different storage buffer formulation.

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NHEDC2 Polyclonal antibody, PBS Only

NHEDC2 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 24065-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24065-1-PBS targets NHEDC2 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NHEDC2 fusion protein Ag21141 相同性解析による交差性が予測される生物種
Full Name Na+/H+ exchanger domain containing 2
Calculated molecular weight 537 aa, 58 kDa
Observed molecular weight ~55 kDa
GenBank accession numberBC047447
Gene Symbol NHEDC2
Gene ID (NCBI) 133308
RRIDAB_2879423
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ86UD5
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

NHEDC2, also named as NHA2 and SLC9B2, contributes to the organellar volume homeostasis. It is required for osteoclast differentiation and bone resorption activity. NHEDC2 has two isoforms with MW 48 kDa and 58 kDa.