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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NISCH Polyclonal antibody, PBS Only

NISCH Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 13813-1-PBS

Synonyms

I1R, I-1 receptor candidate protein, I-1, I 1 receptor candidate protein, I 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NISCH Polyclonal antibody (13813-1-AP)

    WB analysis using 13813-1-AP (same clone as 13813-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13813-1-AP (NISCH antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13813-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of Jurkat cells using NISCH Polyclonal antibody (13813-1-AP)

    IP experiment of Jurkat using 13813-1-AP (same clone as 13813-1-PBS)

    IP result of anti-NISCH (IP:13813-1-AP, 4ug; Detection:13813-1-AP 1:500) with Jurkat cells lysate 1560 ug. This data was developed using the same antibody clone with 13813-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using NISCH Polyclonal antibody (13813-1-AP)

    IP experiment of mouse brain using 13813-1-AP (same clone as 13813-1-PBS)

    IP result of anti-NISCH (IP:13813-1-AP, 4ug; Detection:13813-1-AP 1:500) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 13813-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using NISCH Polyclonal antibody (13813-1-AP)

    IHC staining of human stomach using 13813-1-AP (same clone as 13813-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13813-1-AP (NISCH antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13813-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13813-1-PBS targets NISCH in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NISCH fusion protein Ag4880 相同性解析による交差性が予測される生物種
Full Name nischarin
Calculated molecular weight 1504 aa, 167 kDa
Observed molecular weight 190-200 kDa
GenBank accession numberBC038102
Gene symbol NISCH
Gene ID (NCBI) 11188
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.