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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NIT2 Monoclonal antibody (68169-1-Ig)

    WB analysis using 68169-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68169-1-Ig (NIT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig liver tissue using NIT2 Monoclonal antibody (68169-1-Ig)

    WB analysis of pig liver using 68169-1-Ig

    pig liver tissue were subjected to SDS PAGE followed by western blot with 68169-1-Ig (NIT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using NIT2 Monoclonal antibody (68169-1-Ig)

    WB analysis of mouse liver using 68169-1-Ig

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 68169-1-Ig (NIT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat liver tissue using NIT2 Monoclonal antibody (68169-1-Ig)

    WB analysis of rat liver using 68169-1-Ig

    rat liver tissue were subjected to SDS PAGE followed by western blot with 68169-1-Ig (NIT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using NIT2 Monoclonal antibody (68169-1-Ig)

    WB analysis of HeLa using 68169-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 68169-1-Ig (NIT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using NIT2 Monoclonal antibody (68169-1-Ig)

    IHC staining of human kidney using 68169-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68169-1-Ig (NIT2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using NIT2 Monoclonal antibody (68169-1-Ig)

    IHC staining of human kidney using 68169-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68169-1-Ig (NIT2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using NIT2 Monoclonal antibody (68169-1-Ig)

    IHC staining of human kidney using 68169-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68169-1-Ig (NIT2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using NIT2 Monoclonal antibody (68169-1-Ig)

    IF Staining of U-251 using 68169-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using NIT2 antibody (68169-1-Ig, Clone: 1E5B1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using NIT2 Monoclonal antibody (68169-1-Ig)

    IF Staining of U-251 using 68169-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using NIT2 antibody (68169-1-Ig, Clone: 1E5B1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

NIT2 Monoclonal antibody

NIT2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 68169-1-Ig
Clone No.1E5B1
Publications(1)

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat, pig and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

NIT2, nitrilase family, member 2, Nitrilase homolog 2, Omega amidase NIT2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, pig liver tissue, mouse liver tissue, HepG2 cells, Jurkat cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU-251 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

68169-1-Ig targets NIT2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity Human, mouse, rat, pig
Cited Reactivityhuman
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen NIT2 fusion protein Ag9889 相同性解析による交差性が予測される生物種
Full Name nitrilase family, member 2
Calculated molecular weight 276 aa, 31 kDa
Observed molecular weight31 kDa
GenBank accession numberBC020620
Gene Symbol NIT2
Gene ID (NCBI) 56954
RRIDAB_2923685
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NQR4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NIT2 belongs to a branch of the nitrilase superfamily of enzymes that cleave carbon-nitrogen bonds. NIT2 functions as an omega-amidase and catalyzes hydrolysis of alpha-ketoglutaramate, forming alpha-ketoglutarate and ammonia. This reaction is also functionally coupled with a subset of transaminases that reaminate the keto acid analogs of some essential amino acids, most particularly methionine and phenylalanine.

Protocols

Product Specific Protocols
WB protocol for NIT2 antibody 68169-1-IgDownload protocol
IHC protocol for NIT2 antibody 68169-1-IgDownload protocol
IF protocol for NIT2 antibody 68169-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Commun Biol

Integrating apaQTL and eQTL analysis identifies a potential causal variant associated with lung adenocarcinoma risk in the Chinese population

Authors - Huiwen Xu
View Article