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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NIT2 Monoclonal antibody, PBS Only

NIT2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E5B1

Cat no : 68169-1-PBS

Synonyms

NIT2, nitrilase family, member 2, Nitrilase homolog 2, Omega amidase NIT2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NIT2 Monoclonal antibody (68169-1-Ig)

    WB analysis using 68169-1-Ig (same clone as 68169-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68169-1-Ig (NIT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68169-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig liver tissue using NIT2 Monoclonal antibody (68169-1-Ig)

    WB analysis of pig liver using 68169-1-Ig (same clone as 68169-1-PBS)

    pig liver tissue were subjected to SDS PAGE followed by western blot with 68169-1-Ig (NIT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68169-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using NIT2 Monoclonal antibody (68169-1-Ig)

    WB analysis of mouse liver using 68169-1-Ig (same clone as 68169-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 68169-1-Ig (NIT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68169-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using NIT2 Monoclonal antibody (68169-1-Ig)

    WB analysis of HeLa using 68169-1-Ig (same clone as 68169-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 68169-1-Ig (NIT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68169-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat liver tissue using NIT2 Monoclonal antibody (68169-1-Ig)

    WB analysis of rat liver using 68169-1-Ig (same clone as 68169-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 68169-1-Ig (NIT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68169-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using NIT2 Monoclonal antibody (68169-1-Ig)

    IHC staining of human kidney using 68169-1-Ig (same clone as 68169-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68169-1-Ig (NIT2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68169-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using NIT2 Monoclonal antibody (68169-1-Ig)

    IHC staining of human kidney using 68169-1-Ig (same clone as 68169-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68169-1-Ig (NIT2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68169-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using NIT2 Monoclonal antibody (68169-1-Ig)

    IHC staining of human kidney using 68169-1-Ig (same clone as 68169-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68169-1-Ig (NIT2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68169-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using NIT2 Monoclonal antibody (68169-1-Ig)

    IF Staining of U-251 using 68169-1-Ig (same clone as 68169-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using NIT2 antibody (68169-1-Ig, Clone: 1E5B1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68169-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using NIT2 Monoclonal antibody (68169-1-Ig)

    IF Staining of U-251 using 68169-1-Ig (same clone as 68169-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using NIT2 antibody (68169-1-Ig, Clone: 1E5B1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68169-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68169-1-PBS targets NIT2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity Human, mouse, rat, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen NIT2 fusion protein Ag9889 相同性解析による交差性が予測される生物種
Full Name nitrilase family, member 2
Calculated molecular weight 276 aa, 31 kDa
Observed molecular weight31 kDa
GenBank accession numberBC020620
Gene symbol NIT2
Gene ID (NCBI) 56954
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

NIT2 belongs to a branch of the nitrilase superfamily of enzymes that cleave carbon-nitrogen bonds. NIT2 functions as an omega-amidase and catalyzes hydrolysis of alpha-ketoglutaramate, forming alpha-ketoglutarate and ammonia. This reaction is also functionally coupled with a subset of transaminases that reaminate the keto acid analogs of some essential amino acids, most particularly methionine and phenylalanine.