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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated NKCC1,SLC12A2 Polyclonal antibody

NKCC1,SLC12A2 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-28055

Synonyms

NKCC1, SLC12A2, Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2, BSC2, BSC

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using CoraLite® Plus 488-conjugated NKCC1,SLC12A2 Polycl (CL488-28055)

    IF Staining of U2OS using CL488-28055

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using CoraLite® Plus 488 NKCC1,SLC12A2 antibody (CL488-28055) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-28055 targets NKCC1,SLC12A2 in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NKCC1,SLC12A2 fusion protein Ag27895 相同性解析による交差性が予測される生物種
Full Name solute carrier family 12 (sodium/potassium/chloride transporters), member 2
Calculated molecular weight 131 kDa
Observed molecular weight160 kDa
GenBank accession numberBC033003
Gene symbol NKCC1/SLC12A2
Gene ID (NCBI) 6558
RRIDAB_3672820
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The Na-K-Cl cotransporter sodium-potassium-chloride cotransporter 1 (NKCC1), encoded by the SLC12A2 gene, is an intensively studied member of the CCC family. NKCC1 is a membrane protein and plays fundamental roles in regulating trans-epithelial ion movement, cell volume, chloride homeostasis and neuronal excitability. NKCC1 assembles into a dimer, with the first ten transmembrane (TM) helices harboring the transport core and TM11-TM12 helices lining the dimer interface(PMID: 32081947). The observed molecular weight of NKCC1 is about 140 kDa, glycosylated NKCC1 is detected a band at ∼170 kDa (PMID: 22723696).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 NKCC1,SLC12A2 antibody CL488-28055Download protocol
Standard Protocols
Click here to view our Standard Protocols