TTF1 Monoclonal antibody

TTF1 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG3

Reactivity

human, pig

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

2C8F3

Cat no : 66034-1-Ig

Synonyms

BHC, Homeobox protein Nkx 2.1, NK 2, NK2 homeobox 1, NKX2 1, NKX2.1, NKX2-1, NKX2A, TEBP, Thyroid nuclear factor 1, Thyroid transcription factor 1, TITF1, TTF 1, TTF1


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Tested Applications

Positive WB detected inHepG2 cells
Positive IHC detected inhuman thyroid cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman lung cancer tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66034-1-Ig targets TTF1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Cited Reactivity human
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name NK2 homeobox 1
Calculated molecular weight 42 kDa
Observed molecular weight 41 kDa
GenBank accession numberNM_003317
Gene symbol TTF1
Gene ID (NCBI) 7080
RRIDAB_11045663
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Homeobox protein Nkx-2.1 (NKX2-1), also named Thyroid nuclear factor 1,Thyroid transcription factor 1(TTF1). It is a transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. Crucial in the maintenance of the thyroid differentiation phenotype. May play a role in lung development and surfactant homeostasis. Defects in NKX2-1 are the cause of benign hereditary chorea (BHC), choreoathetosis, hypothyroidism, and neonatal respiratory distress (CHNRD).

Protocols

Product Specific Protocols
WB protocol for TTF1 antibody 66034-1-IgDownload protocol
IHC protocol for TTF1 antibody 66034-1-IgDownload protocol
IF protocol for TTF1 antibody 66034-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

ACS Omega

Interpreting the Pharmacological Mechanisms of Sho-saiko-to on Thyroid Carcinoma through Combining Network Pharmacology and Experimental Evaluation.

Authors - Kun Wang
humanWB

Cell Biochem Biophys

Suberoylanilide Hydroxamic Acid (SAHA) Reduces Fibrosis Markers and Deactivates Human Stellate Cells via the Epithelial-Mesenchymal Transition (EMT).

Authors - Merve Özel
humanIHC

Nat Commun

Single-cell analysis of gastric signet ring cell carcinoma reveals cytological and immune microenvironment features

Authors - Weizhu Zhao