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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using TTF1 Monoclonal antibody (66034-1-Ig)

    WB analysis of HepG2 using 66034-1-Ig (same clone as 66034-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66034-1-Ig (TTF1 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66034-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using TTF1 Monoclonal antibody (66034-1-Ig)

    IHC staining of human lung cancer using 66034-1-Ig (same clone as 66034-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66034-1-Ig (TTF1 antibody at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66034-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using TTF1 Monoclonal antibody (66034-1-Ig)

    IHC staining of human thyroid cancer using 66034-1-Ig (same clone as 66034-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 66034-1-Ig (TTF1 antibody at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66034-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using TTF1 Monoclonal antibody (66034-1-Ig)

    IHC staining of human lung cancer using 66034-1-Ig (same clone as 66034-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66034-1-Ig (TTF1 antibody at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66034-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using TTF1 Monoclonal antibody (66034-1-Ig)

    IHC staining of human lung cancer using 66034-1-Ig (same clone as 66034-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 66034-1-Ig(NKX2-1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66034-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using TTF1 Monoclonal antibody (66034-1-Ig)

    IHC staining of human lung cancer using 66034-1-Ig (same clone as 66034-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 66034-1-Ig(NKX2-1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66034-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using TTF1 Monoclonal antibody (66034-1-Ig)

    IHC staining of human lung cancer using 66034-1-Ig (same clone as 66034-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66034-1-Ig (TTF1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66034-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using TTF1 Monoclonal antibody (66034-1-Ig)

    IHC staining of human lung cancer using 66034-1-Ig (same clone as 66034-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66034-1-Ig (TTF1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66034-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using TTF1 Monoclonal antibody (66034-1-Ig)

    IHC staining of human thyroid cancer using 66034-1-Ig (same clone as 66034-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 66034-1-Ig (TTF1 antibody at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66034-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human lung cancer tissue using TTF1 Monoclonal antibody (66034-1-Ig)

    IF Staining of human lung cancer using 66034-1-Ig (same clone as 66034-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using TTF1 antibody (66034-1-Ig, Clone: 2C8F3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66034-1-PBS in a different storage buffer formulation.

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TTF1 Monoclonal antibody, PBS Only

TTF1 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 66034-1-PBS
Clone No.2C8F3

Host / Isotype

Mouse / IgG3

Reactivity

human, pig

Applications

WB, IHC, IF-P, ELISA

Synonyms

BHC, Homeobox protein Nkx 2.1, NK 2, NK2 homeobox 1, NKX2 1, NKX2.1, NKX2-1, NKX2A, TEBP, Thyroid nuclear factor 1, Thyroid transcription factor 1, TITF1, TTF 1, TTF1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66034-1-PBS targets TTF1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name NK2 homeobox 1
Calculated molecular weight 42 kDa
Observed molecular weight 41 kDa
GenBank accession numberNM_003317
Gene Symbol TTF1
Gene ID (NCBI) 7080
RRIDAB_11045663
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP43699
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

Homeobox protein Nkx-2.1 (NKX2-1), also named Thyroid nuclear factor 1,Thyroid transcription factor 1(TTF1). It is a transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. Crucial in the maintenance of the thyroid differentiation phenotype. May play a role in lung development and surfactant homeostasis. Defects in NKX2-1 are the cause of benign hereditary chorea (BHC), choreoathetosis, hypothyroidism, and neonatal respiratory distress (CHNRD).